法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (4): 250-252.DOI: 10.3969/j.issn.1004-5619.2011.04.003

• 论著 • 上一篇    下一篇

大鼠弥漫性轴索损伤后海马热休克蛋白质70 mRNA表达

汪  枫，李永宏   

  1. （皖南医学院 法医学系，安徽 芜湖 241001）
  • 发布日期:2011-08-25 出版日期:2011-08-28
  • 作者简介:汪枫（1976—），男，安徽芜湖人，讲师，主要从事法医神经病理学研究；E-mail：wangmaster76@163.com

Expression of HSP70 mRNA in Hippocampus of Rats after Diffuse Axonal Injury

WANG FENG, LI YONG-HONG   

  1. （Department of Forensic Medicine, Wannan Medical College, Wuhu 241001, China）
  • Online:2011-08-25 Published:2011-08-28

被引次数

1

摘要： 目的 研究大鼠弥漫性轴索损伤（diffuse axonal injury，DAI）后大脑海马中热休克蛋白质（heat-shock proteins，HSP） 70 mRNA的表达变化。 方法 采用RT-PCR扩增，扩增产物用琼脂糖凝胶电泳检验，观察DAI后大鼠海马中HSP70 mRNA的表达变化。 结果 损伤后4 h，大鼠海马中HSP70 mRNA的表达增加，24 h表达达高峰，48 h后表达减弱。 结论 大鼠海马中HSP70 mRNA表达可作为DAI诊断的参考指标。

关键词: 法医病理学, 弥漫性轴索损伤, 海马, HSP70热休克蛋白质类, 大鼠

Abstract: Objective To explore the changes of expression of heat-shock proteins（HSP） 70 mRNA in hippocampus of rats after diffuse axonal injury（DAI）. Methods RT-PCR products of HSP70 mRNA were tested by agarose gel electrophoresis after RT-PCR amplification. The changes of HSP70 mRNA expression were observed in rat hippocampus after DAI. Results The expression of HSP70 mRNA in the hippocampus could be detected 4 h after DAI. It reached maximum at 24 h and declined after 48 h. Conclusion HSP70 mRNA in hippocampus of rats could be useful for diagnosis of DAI.

Key words: forensic pathology, diffuse axonal injury, hippocampus, HSP70 heat-shock proteins, rats

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