法医学杂志

法医学杂志 ›› 2003, Vol. 0 ›› Issue (1): 24-26.

• 论文 • 上一篇    下一篇

中国5个汉族群体PentaD和PentaE基因座的遗传多态性

刘秋玲,陆惠玲,吕德坚,曾艳红   

  1. 中山大学中山医学院法医物证学教研室,中山大学中山医学院法医物证学教研室,中山大学中山医学院法医物证学教研室,中山大学中山医学院法医物证学教研室 广东广州 510089 ,广东广州 510089 ,广东广州 510089 ,广东广州 510089
  • 发布日期:2003-02-25 出版日期:2003-02-28

Polymorphism of PentaD and PentaE STR locus in five Chinese Han population

LIU QIU-LING, LU HUI-LING,LU DE-JIAN, ET AL. (FACULTY OF FORENSIC MEDICINE, ZHONGSHAN MEDICAL COLLEGE, ZHONGSHAN UNIVERSITY, GUANGZHOU 510089)   

  • Online:2003-02-25 Published:2003-02-28

被引次数

19

摘要： 目的研究PentaD、PentaE基因座在中国广西、湖南、河南、四川、台湾汉族5个群体遗传多态性,比较它们之间的遗传学差异。方法用ProwerPlex(?)16 System荧光标记试剂盒(Promega公司)检测其多态性。结果PentaD、PentaE基因座在5个群体共646名无关个体中分别检出10、19个等位基因,PentaD在湖南汉族首次检出等位基因19。PentaE在河南汉族首次检出等位基因7。PentaD和PentaE在各群体的期望杂合度分别为0.7746～0.8047和0.9005-0.9219,多态信息含量(PIC)为0.7710～0.8025和0.8969-0.9176,个体识别率为0.9223-0.9341和0.9471-0.9782,非父排除率(PE)为0.5435-0.6325和0.6785-0.8465。结论PentaD、PentaE是高度多态性STR基因座,在法医学中有重要应用价值。

关键词: PentaD基因座, PentaE基因座, 遗传多态性

Abstract: Objective To obtain the genetic polymorphism data of Guangxi, Hunan, Henan, Sichuan ,Taiwang Chinese Han population and compare the polymorphism of PentaD and PentaE STR locus. Methods The two loci was analyzed by using the PowerPlex 16 System. Results 10 alleles of PentaD and 19 alleles of PentaE were found in the five Han population. PentaD and PentaE have the expected heterozygosity values of 0.774 6-0.804 7and 0.900 5-0.921 9,the polymorphism information content values of 0.771 0-0.802 5 and 0.896 9-0.917 6, the discrimination power values of 0.9223-0.934 1 and 0.947 1-0.978 2,the power of exclusion values of 0.5435-0.632 5 and 0.678 5-0.846 5, respectively. Conclusion The result showed that these two loci were highly informative and suitable for forensic application.

Key words: PentaD locus, PentaE locus, genetic polymorphism