法医学杂志

法医学杂志 ›› 2003, Vol. 0 ›› Issue (2): 88-91.

• 论文 • 上一篇    下一篇

血中碳氧血红蛋白饱和度测定影响因素的研究

姜宴,叶永红,张宜凡,吴文庆   

  1. 复旦大学上海医学院法医学系,复旦大学上海医学院法医学系,上海市医药学校,复旦大学上海医学院法医学系 上海 200032 ,上海 200032 ,上海 200135 ,上海 200032
  • 发布日期:2003-04-25 出版日期:2003-04-28

Factors affecting the determination of the percent carboxyhemoglobin saturation of blood

JIANG YAN,YE YANG-HONG,ZHANG YI-FAN,ET AL. (DEPARTMENT OF FORENSIC MEDICINE,MEDICAL CENTER OF FUDAN UNIVERSITY,SHANGHAI 200032,CHINA; SHANGHAI PHARMACEUTICAL SCHOOL,SHANGHAI 200135,CHINA)   

  • Online:2003-04-25 Published:2003-04-28

被引次数

13

摘要： 目的 考察血中碳氧血红蛋白饱和度(HbCO％)测定的影响因素,为其结果评定和所需样品保存条件提供实验依据。方法 利用三种分光光度法,测定30d内不同条件下保存的CO阳性血的HbCO％的变化。结果 还原双波长法、双波长法测定结果比较稳定,单波长法抗干扰能力较差;尸检所取血样的保存条件包括温度、保存时间及与空气接触程度对HbCO％的测定均有影响,其中温度影响较为显著。结论 利用还原双波长法与双波长法,并结合光谱扫描观察峰形变化可得到比较可靠的结果。30d内4℃条件下,密闭容器中血样接触少量空气不影响其HbCO％的测定。

关键词: HbCO饱和度, 分光光度法, 样品保存条件

Abstract: Objective To investigate factors affecting the determination of the percent carboxyhemoglobin saturation (HbCO%) of blood in an attempt to offer further data for results interpretation and sample storage requirement. Methods The HbCO% of blood samples stored in various conditions were detected by three spec-trophotometries during the succeeding 30 days. Results The data detected by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry were more stable than mono-wavelength spec-trophotometry. The HbCO% of blood was significantly related with the storage conditions which include temperature,time and the degree of exposure to air. Conclusion Determinations of HbCO% are reliable which performed by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry,combine with spectral scans. During 30 days,blood stored at 4℃ exposed to a limited volume of air does not influence the determination of HbCO%.

Key words: percent carboxyhemoglobin saturation, spectrophotometry, sample storage condition