法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (1): 1-3+69.

• 论文 •    下一篇

皮肤切创愈合中caspase-3表达的免疫组化研究

杜宇,官大威,赵锐   

  1. 中国医科大学法医学院,中国医科大学法医学院,中国医科大学法医学院 辽宁沈阳110001 ,辽宁沈阳110001 ,辽宁沈阳110001
  • 发布日期:2004-02-25 出版日期:2004-02-28

An immunohistochemical study on the expression of caspase-3 in the skin wound healing

DU YU,GUAN DA-WEI,ZHAO RUI(SCHOOL OF FORENSIC MEDICINE,CHINA MEDICAL U NIVERSITY,SHENYANG110001,CHINA)   

  • Online:2004-02-25 Published:2004-02-28

被引次数

73

摘要： 目的探讨皮肤切创损伤愈合过程中,caspase-3在损伤区内的表达以及不同损伤时间caspase-3的变化规律。方法应用免疫组织化学技术对33例不同损伤时间小鼠皮肤切创组织中caspase-3的表达进行研究。同时以3例非切创小鼠皮肤组织做对照。结果伤后6h的损伤皮肤组织中可见少量中性粒细胞表达caspase-3,伤后12～24h,大部分浸润的中性粒细胞及部分单核细胞为caspase-3阳性。随伤后时间延长,caspase-3阳性细胞以单核细胞及成纤维细胞为主。伤后0～3h,caspase-3阳性细胞比率较低,为(4.53±6.53)%,12h后逐渐增加,伤后3d达高峰,为(62.66±4.84)%,其后逐渐下降。结论小鼠皮肤损伤愈合过程中,caspase-3可能在诱导损伤区内中性粒细胞、单核巨噬细胞及成纤维细胞发生凋亡过程中发挥重要作用,同时,caspase-3的规律性表达可用于损伤时间的推断。

关键词: 皮肤损伤, 凋亡, 损伤时间推断, caspase-3, 免疫组织化学

Abstract: Objective To investigate the expressi on of caspase-3during skin wound healing and explore the applicability of caspase-3to determination of wound age.Methods The expression of caspase-3were performed on33mice skin incised wounds at different posttraumatic intervals and3mice as c ontrol by immunohistochemical technique.Results Expression of caspase-3was detec table in polymorphonuclear cells(PMNs)in the wound specimens aged6h.In the wound specimens aged from12h to24h after injury,caspase-3was identified in a large nu mber of infiltrating PMNs and part of mononuclear cells(MNCs).Afterwards,the MNC s and fibroblastic cells(FBCs)accounted in the most part of the caspase-3positiv e cells.Morphometrically,the ratio of the number of the caspase-3stained PMNs,MN Cs and FBCs to total number of the cells in the wounds was evaluated and calcula ted.The ratio of the caspase-3positive cells was low in the wound specimens aged from0h to3h(4.53 ± 6.53)%,and maximized in the wound speci-mens aged3day(62.66 ± 4.84)%.Thereafter,the ratio decreased and minimized in the specimens aged14d ay(21.56 ± 4.54)%.Conclusion The results suggest that caspase-3may play an important ro le in inducing neutrophil,macrophage and fibroblast apoptosis during skin wound healing.Furthermore,caspase-3may be used as a marker for the wound age determination.

Key words: skin wound, apoptosis, wound age determination, caspase-3, immunohis tochemistry