法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (2): 120-123.

• 论文 • 上一篇    下一篇

HLA基因分型技术的进展与展望

李成涛   

  1. 司法部司法鉴定科学技术研究所 上海200063
  • 发布日期:2004-04-25 出版日期:2004-04-28

Progress and prospects of HLA genotyping technology

LI CHENG-TAO (INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE, P.R.CHINA, SHANGHAI 200063, CHINA)   

  • Online:2004-04-25 Published:2004-04-28

被引次数

46

摘要： 研究人的主要组织相容性复合物HLA(humanleucocyteantigen,人类白细胞抗原)的结构和功能有助于认识生命现象的本质以及疾病的致病机理,因此长期以来一直是生命科学研究的重点之一。随着分子生物学技术的迅速发展,HLA分型已经由传统的血清学水平向DNA水平过渡,并取得了很好的效果。本文对目前各种HLA基因分型方法的原理和优缺点结合自己的应用经验做了评价,并简要介绍了在HLA基因分型研究中出现的新的动态。

关键词: 人类白细胞抗原, 基因分型, 分型标准化

Abstract: Over a long period of time, studies on HLA structure and function have been the research hotspots. for it is very important to understand the essential of life science and disease mechanism. With the rapid development of molecular biology, HLA typing makes great progress. It has changed from traditional serological typing to DNA-based typing. More and more HLA genotyping methods have been developed and applied. In this essay, the author reviewed and appraised all kinds of HLA genotyping tech niques and introduced two new techniques.

Key words: human leucocyte antigen, genotyping, standardized DNA-based typing