法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (2): 156-158.

• 论文 • 上一篇    下一篇

降解生物检材遗传标记检测的研究进展

何柏芳;楼迪栋;   

  1. 华中科技大学同济医学院法医学系01级,温州医学院法医学系 湖北武汉430030,浙江温州325000
  • 发布日期:2006-04-25 出版日期:2006-04-28

A Reviewing for Profiling of Highly Degraded Remains

HE BAI-FANG1, LOU DI-DONG2 (1.DEPARTMENT OF FORENSIC MEDICINE, TONGJI MEDICAL COLLEGE, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY,WUHAN 430030,CHINA; 2.DEPARTMENT OF FORENSIC MEDICINE,WHENZHOU MEDICAL COLLEGE,WHENZHOU 325000,CHINA )   

  • Online:2006-04-25 Published:2006-04-28

被引次数

5

摘要： 降解生物检材遗传标记的检测一直是法医学实践和科研的难点。虽然PCR-STR分析技术能在一定程度上检测降解生物检材的遗传标记,但是对于某些高度降解的生物检材如骨骼、牙齿等的PCR-STR分型仍旧十分困难,存在不稳定性和分型异常现象。本文就近几年来PCR-STR分型技术在降解生物检材的法医实践中出现的问题和解决方法作一综述。

关键词: 降解生物检材, 扩增不平衡, 等位基因丢失, DNA分型异常

Abstract: It′s always a challenge to type from highly degraded biological remains. PCR-based STR genotyping is helpful and valuable for such degraded samples like bones, teeth et al, but the typing results are sometimes unstable or wrong. Here the methods for solving the problems and improving reproducibility are reviewed.

Key words: degraded biological remains, amplification inequality, allele loss, DNA typing anomaly