法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (3): 196-197+.

• 论文 • 上一篇    下一篇

激光显微捕获口腔上皮细胞的DNA分型

顾丽华;张晨;陈连康;郑会芬;程莉;周怀谷;   

  1. 上海市刑事科学技术研究所,上海市刑事科学技术研究所,上海市刑事科学技术研究所,上海市刑事科学技术研究所,上海市刑事科学技术研究所,上海市刑事科学技术研究所 上海200083,上海200083,上海200083,上海200083,上海200083,上海200083
  • 发布日期:2006-06-25 出版日期:2006-06-28

DNA Genotyping of Oral Epithelial Cells by Laser Capure Microdissection

GU LI-HUA, ZHANG CHENG, CHEN LIAN-KANG, ZHEN HUI-FEN, CHENG LI, ZHOU HUAI-GU(SHANGHAI INSTITUTE OF FORENSIC SCIENCE,SHANGHAI, 200083,CHINA)   

  • Online:2006-06-25 Published:2006-06-28

摘要： 目的探索激光显微技术(lasercapturemicrodissectionsystem,LCM)捕获口腔上皮细胞,并进行STR-DNA分型检测的方法。方法用VERITAS显微切割仪红外低能激光显微捕获一定数量口腔上皮细胞,进行ProfilerPlus试剂盒STR复合扩增,检测DNA基因型。结果7～8个口腔上皮细胞能成功获得STR-DNA分型。3～4个口腔上皮细胞不能成功获得STR-DNA分型。结论激光显微捕获作为一种分离单个细胞的新技术,对于微量口腔上皮细胞的STR-DNA分型是可行的。

关键词: 激光显微捕获(LCM), 口腔上皮细胞, STR-DNA分型

Abstract: objective The STR genotypping of trace oral epithelial cells which are microdissected by laser capure microdissection system(LCM) is explored. Methods The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures. Results DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed. Conclusion It is viable in genotyping of trace oral epithelial cells by Laser Capure Microdissection as a new technology of seperating single cell.

Key words: laser capure microdissection(LCM), oral epithelial cells, STR-DNA typing