法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (5): 324-327.

• 论文 • 上一篇    下一篇

闭合性颅脑损伤后大鼠BAEP及脑损伤的动态变化

吴敬杰;刘敏;闫红涛;郑重;王晔;李斌;廖志钢;   

  1. 四川大学华西基础医学与法医学院法医病理学教研室,四川大学华西基础医学与法医学院法医病理学教研室,四川大学华西基础医学与法医学院法医病理学教研室,成都中医药大学临床医学院,四川大学华西基础医学与法医学院法医病理学教研室,四川大学华西基础医学与法医学院法医病理学教研室,四川大学华西基础医学与法医学院法医病理学教研室 四川成都610041贵州警官职业学院实验中心,贵州贵阳550005,四川成都610041,四川成都610041,四川成都610075,四川成都610041,四川成都610041,四川成都61004
  • 发布日期:2006-10-25 出版日期:2006-10-28

Dynamic Observation of Brainstem Auditory Evoked Potential and Pathological Changes of the Brain after Closed Brain Injuries in Rats.

WU JING-JIE1,2, LIU MING1, YAN HONG-TAO1, ZHENG ZHONG3, WANG YE1, LI BIN1, LIAO ZHI-GANG1 (1.DEPARTMENT OF FORENSIC PATHOLOGY, WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041,CHINA; 2. EXPERIMENTAL CENTER OF GUIZ   

  • Online:2006-10-25 Published:2006-10-28

被引次数

8

摘要： 目的观察大鼠闭合性弥漫性颅脑损伤后脑干听觉诱发电位(BAEP)的动态改变及脑组织病理学变化,探讨BAEP在颅脑损伤后评估听觉功能障碍的价值。方法使用自制弹簧式小型生物打击机打击大鼠颅顶部,制造闭合性弥漫性轻型颅脑损伤模型。观察对照组以及伤后15min和1,3,6,12h及1,2,4,7,10,14,21d等时间点大鼠脑组织病理学改变,干/湿法检测脑组织含水量。分别于伤前、伤后各时间点以50Hz刺激率记录大鼠BAEP,对其结果进行比较。结果损伤后15min,BAEP的Ⅰ～Ⅴ、Ⅲ～ⅤIPL即较损伤前延长(P<0.05),至6～12h,Ⅲ、ⅤPL较损伤前延长。损伤后1～2d,Ⅲ、ⅤPL和Ⅰ～Ⅲ、Ⅰ～Ⅴ、Ⅲ～ⅤIPL均较损伤前显著延长(P<0.001),14d后BAEP逐渐恢复正常。伤后15min脑组织含水量开始升高,伤后1d达高峰,持续至4d后逐渐下降,至10d后降至正常水平。结论BAEP可作为闭合性弥漫性轻型颅脑损伤后听觉功能障碍的客观评价指标。

关键词: 轻型颅脑损伤, 闭合性, 病理学, 脑干听觉诱发电位, 大鼠

Abstract: Objective To investigate the dynamic experiences of pathological changes in brain and the injured role of brainstem auditory evoked potential (BAEP) after closed Mild Traumatic Brain Injury (MTBI) in rat. Methods A closed diffuse brain injury model was successfully produced in rat by hit the parietal bone with a spring-droved steel stick. The auditory brainstem response evoked by click at 50 Hz stimulating frequency were recorded at pre-trauma, 15 min and 1,3,6 ,12 h and 1,2,4,7,10,14,21 d after brain injury. The pathological changes of brain were observed under light microscope and the brain edema was detected by wet weight/dry weight rations. Results The Ⅰ-Ⅴ, Ⅲ-Ⅴof BAEP wave inter peak latency (IPL) in MTBI group increased immediately in 15 minutes after injury. The Ⅲ,Ⅴ wave peak latency (PL) and Ⅰ-Ⅴ, Ⅲ-Ⅴwave IPL became longer than the pre-trauma level in 6, 12 h post-injury (P<0.05). The Ⅲ,Ⅴ wave PL and Ⅰ-Ⅲ,Ⅰ-Ⅴ, Ⅲ-Ⅴ wave IPL became longer than the pre-trauma level in 1, 2 d after injury(P<0.001), the BAEP retuned to normal level in 14 d after brain injury. At 15 minutes after injury, the water content in brain increased and met its peak in 1 d, decreased gradually in 4 d, then decreased slowly and returned to normal level in 10 d after injury. Conclusion The regular changes of BAEP could be an objective tool for evaluating hearing dysfunction after closed mild diffuse traumatic brain injury.

Key words: mild traumatic brain injury, closed, pathology, brainstem auditory evoked potential(BAEP), rat