法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (2): 94-96+1.

• 论文 • 上一篇    下一篇

人皮肤成纤维细胞早期损伤后EDA-FN表达与损伤时间的关系

贺盟;余松;薛爱民;沈忆文;陈龙;顾云菊;赵子琴;   

  1. 复旦大学上海医学院法医系,湖州市公安局,复旦大学上海医学院法医系,复旦大学上海医学院法医系,复旦大学上海医学院法医系,复旦大学上海医学院法医系,复旦大学上海医学院法医系 上海200032,浙江湖州313000,上海200032,上海200032,上海200032,上海200032,上海200032
  • 发布日期:2007-04-25 出版日期:2007-04-28

Expression of EDA-fibronectin in Injured Human Skin Fibroblast and its Potential Application in Estimation of Wound Healing

HE MENG1,YU SONG2,XUE AI-MIN1,SHEN YI-WEN1,CHEN LONG1,GU YUN-JU1,ZHAO ZI-QIN1(DEPARTMENT OF FORENSIC MEDICINE,SHANGHAI MEDICAL COLLAGE,FUDAN UNIVERSITY,SHANGHAI 200032,CHINA)   

  • Online:2007-04-25 Published:2007-04-28

摘要： 目的探讨培养的人体皮肤成纤维细胞损伤后纤维连接蛋白EDA片段的表达情况与损伤时间的关系。方法培养人体皮肤成纤维细胞,利用单克隆抗体进行免疫组织化学染色及Western检测。结果培养中的人体成纤维细胞可以微量表达EDA,随损伤时间延长而表达量增加。结论EDA片段表达变化可以作为法医学早期损伤时间推断的比较理想的指标。

关键词: 纤维连接蛋白, EDA片段, 损伤时间, 成纤维细胞

Abstract: Objetive To study the expression of EDA-FN variant in injured human skin fibroblast and its relation to wound healing.Methods Human skin fibroblast cells were separated from human skin and cultured and monoclonal antibody to fibronectin [IST-9] was applied to amplify target segments.Results Trace amount of EDA-FN was detected in the culture cells.The expression was increased within 8 hours after the injury.Conclusions The expression of EDA-FN would be an ideal marker for estimation of wound healing

Key words: FN, EDA, wound interval estimation, fibroblast