法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (4): 245-247+.

• 论文 • 上一篇    下一篇

电击死兔骨骼肌及心肌HSP 70 mRNA和c-fos mRNA表达

王晔;刘敏;程薇波;何桂琼;李凡;廖志钢;   

  1. 四川大学华西基础医学与法医学院;四川大学华西公共卫生学院;
  • 发布日期:2008-08-25 出版日期:2008-08-28

The Expressions of HSP 70 mRNA and c-fos mRNA in the Skeletal Muscle and Cardiac Muscle of Rabbits by Electrocuted

WANG YE1,2,LIU MIN1,CHENG WEI-BO2,HE GUI-QIONG1,LI FAN1,3,LIAO ZHI-GANG1(1.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE,SICHUAN UNIVERSITY,CHENGDU 610041,CHINA;2.WEST CHINA SCHOOL OF PUBLIC HYGIENICS,SICHUAN UNIVERSITY,CHENGDU 610041,CHINA;3.HEN   

  • Online:2008-08-25 Published:2008-08-28

被引次数

14

摘要： 目的研究电击死兔骨骼肌与心肌HSP 70 mRNA和c-fos mRNA表达变化,探究生前电击与死后电击的鉴别方法。方法15只新西兰兔,随机分电击死组、死后电击组和对照组,每组5只,用荧光RT-PCR技术检测骨骼肌与心肌热休克蛋白70(HSP 70)mRNA与c-fos mRNA表达水平,对所得结果进行统计学分析。结果生前电击兔骨骼肌及心肌HSP 70 mRNA与c-fos mRNA表达高于死后即刻电击者(P<0.05)。结论检测骨骼肌及心肌HSP 70 mRNA与c-fos mRNA表达变化有助于于生前电击与死后电击的鉴别。

关键词: 法医病理学, 电击伤, 荧光光度测定法, HSP 70热休克蛋白质类, 基因, fos, 逆转录聚合酶链反应

Abstract: Objective To study the changes of HSP 70 mRNA and c-fos mRNA expression and to find a method to differentiate antemortem from postmortem electrocution.Methods Fifteen New Zealand rabbits were randomly divided into three groups,the antemortem electrocution group,the postmortem electrocution group,and the control group.Each group consists of five rabbits.The levels of HSP 70 mRNA and c-fos mRNA in skeletal muscle and cardiac muscle were examined with quantitative fluorescent RT-PCR.Results The levels of HSP 70 mRNA and c-fos mRNA in the antemortem electrocution group increased significantly(P<0.05),compared with that of the postmortem electrocution group.Conclusion The changes of HSP 70 mRNA and c-fos mRNA expression in skeletal muscle and cardiac muscle can be used as an indicator to distinguish antemortem from postmortem electrocution.

Key words: forensic pathology, electric injuries, flurphotometry, HSP 70 heat-shock proteins, genes, fos, reverse transcriptase polymerase chain reaction