法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (4): 262-264+.

• 论文 • 上一篇    下一篇

利用荧光AFLP技术检测罂粟DNA多态性

路帆;程宝文;李虹;洪俊杰;孙海燕;赵文嵩;杨红梅;李进;赵郁荣;   

  1. 云南省公安厅刑事科学技术研究所;昆明明技科技有限公司;普洱市公安局刑事科学技术研究所;临沧市公安局刑事科学技术研究所;
  • 发布日期:2008-08-25 出版日期:2008-08-28

DNA Polymorphism Detection of Papaver Somniferum L Using Fluorescent Amplified Fragment Length Polymorphism

LU FAN1,CHENG BAO-WEN1,LI HONG1,HONG JUN-JIE2,SUN HAI-YAN2,ZHAO WEN-SONG1,YANG HONG-MEI1,LI JIN3,ZHAO YU-RONG4(1.INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY,YUNNAN PROVINCE POLICE BUREAU,KUNMING 650021,CHINA;2.KUNMING MINGJI SCIENCE AND TECHNOLOGY CO.,LT   

  • Online:2008-08-25 Published:2008-08-28

被引次数

7

摘要： 目的利用荧光AFLP检测技术检测罂粟植物DNA多态性。方法用Axygen公司的AxyPrep DNA试剂盒提取了12株产于缅甸和中国云南省昆明市宜良县罂粟植株的DNA,用EcoRI和MseI对总DNA进行酶切,连接人工接头,预扩增和选择性扩增,其产物在CEQ8000遗传分析系统上检测。结果64对选择性扩增引物中8对引物能得到20条以上的扩增片断,具有高度多态性。结论荧光AFLP技术可用于罂粟DNA多态性的检测。

关键词: 法医学, 植物学, 罂粟, 扩增片段长度多态性

Abstract: Objective To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.Methods Genomic DNA was isolated using the AxyPrep DNA Kit,double-digested by two restrictional endonucleases(Eco RI and Mse I) and ligated to oligonucleotide adapters.After Pre-amplification and selective amplification,the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.Results More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.Conclusion The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.

Key words: forensic medicine, botany, papaver somniferum L, amplified fragment length polymorphism