法医学杂志

法医学杂志 ›› 2009, Vol. 25 ›› Issue (2): 81-84.

• 论文 • 上一篇    下一篇

大鼠肌肉中微生物ATP含量及其在死亡时间推断中的应用

刘茜;翁义星;杨帆;郑娜;刘良;   

  1. 华中科技大学同济医学院法医学系;福州市公安局刑侦支队;中国政法大学证据科学教育部重点实验室;
  • 发布日期:2009-04-25 出版日期:2009-04-28

Microbial ATP in Rat Muscle Tissue and Its Application for the Estimation of Postmortem Interval

LIU QIAN1, WENG YI-XING2, YANG FAN1, ZHENG NA1, LIU LIANG1,3 (1. DEPARTMENT OF FORENSIC MEDICINE, TONGJI MEDICAL COLLEGE, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY, WUHAN 430030, CHINA; 2. PUBLIC SECURITY BUREAU OF FUZHOU, FUZHOU 350011, CHINA; 3. KEY   

  • Online:2009-04-25 Published:2009-04-28

被引次数

12

摘要： 目的研究死后大鼠肌肉组织中微生物ATP浓度变化及其与死亡时间(postmortem interval,PMI)的关系。方法取健康SD大鼠处死,死后不同时间取肌肉组织,用生物发光法检测微生物ATP浓度,对所得数据进行统计学处理。结果死后肌肉组织中微生物ATP含量随时间延长而增加,于7d达到高峰,随后逐渐降低,并于10d再次略微升高。PMI在0～9d内与肌肉中微生物ATP含量变化拟合最优。以PMI为自变量的三次多项式回归方程为:y=0.020x3-0.166x2-0.666x+13.412(r2=0.989,P<0.01)。结论死后肌肉组织中微生物ATP含量变化可用于PMI推测;本检测方法所需检材量小,不受组织自身降解的影响。

关键词: 法医病理学, 腺苷三磷酸, 发光测定法, 死亡时间, 微生物

Abstract: Objective To study the relationship between the changes of microbial ATP in rat muscle tissue and the postmortem interval(PMI). Methods Healthy SD rats were sacrificed and their muscles were sampled at different postmortem intervals. The concentration of microbial ATP was detected using bioluminescent assay and the data was statistically analyzed. Results The content of microbial ATP in rat muscle tissue increased along with PMI extension and peaked on postmortem day 7, thereafter decreased gradually, but increased slight- ly on postmortem day 10 once again. The PMI correlated best with the content of microbial ATP in rat muscle tissue within 9 days. If the PMI was the independent variable, the cubic polynomial regression equa- tion was y=0.020 x3-0.166 x2-0.666 x+13.412(r2=0.989, P<0.01). Conclusion Changes of microbial ATP content in rat muscle tissue may be used for estimation of PMI. Since this assay requires only small amount of tissue with much less influence by self-decomposition, it may broaden the time range of PMI estimation.

Key words: forensic pathology, adenosine triphosphate, luminescent measurements, postmortem interval, microbes