法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (4): 249-253.

• 论文 • 上一篇    下一篇

大鼠脑震荡伤后脑组织HSP70、bFGF及TGF-β1表达变化

陈锐;余彬华;胡玲;严治;王文东;刘敏;   

  1. 四川大学华西基础医学与法医学院;广东医学院法医学教研室;峨眉山市公安局;广东南天司法鉴定所;
  • 发布日期:2009-08-25 出版日期:2009-08-28

Changes of HSP70, bFGF and TGF-β1 Expression in Rat Brain after Concussion

CHEN RUI1,2, YU BIN-HUA3, HU LING1,4, YAN ZHI1, WANG WEN-DONG1, LIU MIN1 (1. WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041, CHINA; 2. DEPARTMENT OF FORENSIC MEDICINE, GUANGDONG MEDICAL COLLEGE, DONGGUAN 523808,   

  • Online:2009-08-25 Published:2009-08-28

被引次数

10

摘要： 目的研究脑震荡后脑组织损伤修复相关因子表达变化,为推断脑损伤时间提供法医学依据。方法建立SD大鼠脑震荡伤模型,提取脑组织行常规HE染色及热休克蛋白70(heat shock protein 70,HSP70)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)和转化生长因子β1(transforming growth factor beta1,TGF-β1)免疫组织化学染色,在光学显微镜下观察并进行图像分析及相应统计学处理。结果伤后30min,在大脑皮质、海马区见少量呈弥散性分布的HSP70免疫组化染色阳性细胞,12h达高峰,伤后1d阳性细胞数量开始减少;伤后3h,在大脑皮质、海马区bFGF免疫组化染色阳性细胞开始增多,至12h时达高峰,然后开始下降;伤后6h～1d,在大脑皮质、海马区TGF-β1免疫组化染色阳性细胞逐渐增多,至3d时达高峰,然后开始下降。结论HSP70、bFGF、TGF-β1在脑震荡后脑组织中表达呈时序性改变。多指标综合应用为法医学损伤时间的推断提供了全新的视角。

关键词: 法医病理学, 脑震荡, HSP70热休克蛋白质类, 成纤维细胞生长因子, 转化生长因子β1, 损伤时间的推断, 大鼠

Abstract: Objective To study the changes of expression of relevant factors in rat brain after concussion injury and to provide scientific basis for forensic estimation of brain injury interval. Methods Brain tissues were sampled from the established SD rat animal model of brain concussion, routinely processed and stained with HE and immunohistochemically stained with antibodies directed against heat shock protein 70 (HSP 70), transforming growth factor beta 1(TGF-β1) and basic fibroblast growth factor(bFGF). The sections were examined under light microscope with IMAGE analytical system and homologous statistical analysis. Results The expression of HSP 70 was observed in 30 minutes after brain injury. The amount of neurons expressing HSP 70 increased gradually, reached its peak at 12 hours and then declined at 24 hours after brain injury. The expression of bFGF was observed 3 hours after injury in brain stem, reached its peak at 12 hours, and then declined. The expression of TGF-β1 was detected 6-24 hours after brain injury, remained at its peak up to 3 days. Conclusion Brain injury can induce a chronological expression of HSP 70, bFGF and TGF-β1. The results can be a potential for estimating the age of brain injury using several markers.

Key words: forensic pathology, brain concussion, HSP 70 heat-shock proteins, transforming growth factor beta1, fibroblast growth factor, wound age estimation, rats