法医学杂志

法医学杂志 ›› 2010, Vol. 0 ›› Issue (1): 15-17.

• 论文 • 上一篇    下一篇

大鼠脑震荡后c-jun蛋白表达变化

汪枫;李永宏;   

  1. 皖南医学院法医学系;
  • 发布日期:2010-02-25 出版日期:2010-02-28

Expression of c-jun Protein after Experimental Rat Brain Concussion

WANG FENG,LI YONG-HONG(DEPARTMENT OF FORENSIC MEDICINE,WANNAN MEDICAL COLLEGE,WUHU 241001,CHINA)   

  • Online:2010-02-25 Published:2010-02-28

被引次数

1

摘要： 目的观察实验性大鼠脑震荡后c-jun蛋白的表达变化规律,探索脑震荡后法医病理学的诊断指标。方法将55只大鼠随机分为脑震荡组和对照组。用免疫组织化学SABC法观察大鼠脑震荡后脑内c-jun蛋白表达变化的规律。结果对照组大鼠可见c-jun蛋白的弱阳性表达。脑震荡组损伤后15min可在神经元观察到c-jun蛋白的表达,随着损伤后经过时间的延长,表达c-jun蛋白的细胞数及范围逐渐扩大,损伤后3h表达达高峰。结论c-jun蛋白的检测可作为诊断脑震荡和推断脑震荡后经过时间的一项敏感指标。

关键词: 法医病理学, 脑震荡, 原癌基因蛋白质c-jun, 损伤时间, 大鼠

Abstract: Objective To observe c-jun protein expression after rat brain concussion and explore the forensic pathologic markers following brain concussion.Methods Fifty-five rats were randomly divided into brain con-cussion group and control group.The expression of c-jun protein was observed by immunohistochemistry.Results There were weak positive expression of c-jun protein in control group.In brain concussion group,however,some neutrons showed positive expression of c-jun protein at 15 min after brain concussion,and reach to the peak at 3 h after brain concussion.Conclusion The research results suggest that detection of c-jun protein could be a marker to determine brain concussion and estimate injury time after brain concussion.

Key words: forensic pathology, brain concussion, proto-oncogene proteins c-jun, wound age, rats