法医学杂志

法医学杂志 ›› 2010, Vol. 0 ›› Issue (2): 84-87.

• 论文 • 上一篇    下一篇

外伤性癫痫灶中泛素与泛素激活酶的定量分析

何鹏翔;唐运林;蔡卫林;黄永华;方超;竞花兰;   

  1. 中山大学中山医学院法医系;上海市公安局金山分局;中国人民解放军广州空军医院;
  • 发布日期:2010-04-25 出版日期:2010-04-28

The Quantitative Analysis of Ub and UbE1 in Focus of Traumatic Epilepsy

HE PENG-XIANG1,3,TANG YUN-LIN2,CAI WEI-LIN1,HUANG YONG-HUA1,FANG CHAO1,JING HUA-LAN1 (1. DEPARTMENT OF FORENSIC MEDICINE,ZHONGSHAN MEDICAL COLLEGE,SUN YAT-SEN UNIVERSITY,GUANGZHOU 510080,CHINA; 2. THE PLA AIR FORCE HOSPITAL OF GUANGZHOU,GUANGZHOU 510602,C   

  • Online:2010-04-25 Published:2010-04-28

被引次数

1

摘要： 目的定量测定人体外伤性癫痫灶中泛素(ubiquitin,Ub)和泛素激活酶(ubiquitin-activating en-zyme,UbE1)的表达,研究泛素蛋白酶体系在外伤性癫痫(post-traumatic epilepsy,PTE)发病机制中所起的作用,探索其在PTE鉴定中的应用价值。方法分别收集颅脑外伤致癫痫患者脑癫痫灶组织(外伤性癫痫组)、非颅脑外伤性癫痫灶组织(非外伤性癫痫组)和交通事故死亡者正常脑组织(非癫痫组)各15例,应用RT-PCR、Western印迹技术检测3组中Ub和UbE1的含量,并对所得数据进行统计学分析。结果RT-PCR、Western印迹技术测定显示Ub和UbE1的mRNA和蛋白质在3组中含量为外伤性癫痫组>非外伤性癫痫组>非癫痫组,两两比较,组间差异具有统计学意义(P<0.05)。结论Ub和UbE1在癫痫灶组织中表达增加,且在外伤性癫痫灶组织中增加最为明显。推断泛素蛋白酶体系的活化是PTE神经元病理改变的重要机制之一。

关键词: 法医病理学, 癫痫, 创伤后, 泛素, 泛素激活酶, 逆转录聚合酶链反应, 印迹法, 蛋白质

Abstract: Objective To study the expression of ubiquitin proteasome system(UPS) in the traumatic epilepsy pathogenesis and its value in traumatic epilepsy by quantitative analysis. Methods Fifteen specimens from human epileptic temporal cortex from PTE were collected as the PTE group. Fifteen specimens from non-PTE were collected as the non-PTE group. Fifteen normal cerebral cortex specimens died from acute traffic accident were collected as the control group. The expression of mRNA and protein of Ub and UbE1 were detected by RT-PCR and Western blot. Statistical analysis was used to compare the data between three groups. Results The expression of mRNA and protein of Ub and UbE1 were the following order:PTE group(high),non-PTE group(middle) and control group(low). Conclusion The study confirms that UPS is up-regulated in the epilepsy's focus,especially in traumatic epilepsy. The activation of UPS may be an important pathological change in neurons in pathogenesis of traumatic epilepsy.

Key words: forensic pathology, epilepsy, post-traumatic, ubiquitin(Ub), ubiquitin-activating enzyme(UbE1), reverse transcriptase polymerase chain reaction(RT-PCR), blotting, Western