法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (3): 161-164.

• 论文 •    下一篇

小鼠死后骨骼肌、心、肝、肾、脑细胞核中DNA的变化规律

郑吉龙;李晓娜;张晓东;牛青山;   

  1. 中国医科大学法医学院;中国刑事警察学院法医系;中国医科大学基础医学院;
  • 发布日期:2010-06-25 出版日期:2010-06-28

DNA Degradation in Nucleolus of Skeletal Muscle, Heart, Liver, Kidney and Brain in Mice after Death

ZHENG JI-LONG1,2,LI XIAO-NA3,ZHANG XIAO-DONG2,NIU QING-SHAN2(1.SCHOOL OF FORENSIC MEDICINE,CHINA MEDICAL UNIVERSITY,SHENYANG 110001,CHINA;2.DEPARTMENT OF FORENSIC MEDICINE,CHINA CRIMINAL POLICE UNIVERSITY,SHENYANG 110035,CHINA;3.COLLEGE OF BASIC MEDICAL S   

  • Online:2010-06-25 Published:2010-06-28

摘要： 目的研究小鼠器官细胞核中DNA降解变化与死亡时间的关系,为法医学推断死亡时间提供一种新方法。方法应用单细胞凝胶电泳技术结合荧光显微镜和计算机图像分析技术,测定小鼠在死后72h内不同时间点骨骼肌、心、肝、肾、脑细胞核头DNA含量、尾DNA含量、头半径、尾长度、尾矩、Olive矩、头面积、尾面积8项参数的变化值。结果在小鼠死亡72h内,彗星尾DNA含量、尾长度、尾矩、Olive矩、尾面积都呈增加趋势,头DNA含量、头半径、头面积均呈下降趋势。计算出体现DNA降解趋势的二项式回归方程(P<0.001)和多元回归方程(P<0.0001)。结论本研究提供的各组回归方程,可为法医学推断死后经过时间提供一种新的方法。

关键词: 法医病理学, 细胞核, DNA降解, 死亡时间推断, 小鼠

Abstract: Objective To study the change of DNA degradation in nucleolus of mice organs and its relationship with the postmortem interval,and to investigate a new accurate method to estimate the postmortem interval.Methods Eight parameters of cell nuclei were chosen,including the head DNA level,the tail DNA level,the head radius,the tail length,the tail moment,the Olive moment,the head area and the tail area.The changes of DNA degradation were analyzed in skeletal muscle,myocardium,liver,kidney and brain in mice at different intervals(0-72 h postmortem) by using single-cell gel electrophoresis and fluorescent microscope connected with auto-analysis-image system.Results The tail DNA level,the tail length,the tail moment,the Olive moment and the tail area showed an increasing tendency.The head DNA level,the head radius and the head area showed a decreasing tendency within 72 h postmortem in mice.A quadratic regression equation(P<0.001) and multiple regression equation of DNA degradation tendency were established(P<0.000 1).Conclusion The regression equations established can be used as a new method for estimating postmortem interval in forensic practice.

Key words: forensic pathology, cell nucleus, DNA degradation, postmortem interval estimation, mice