法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (1): 9-12,21.DOI: 10.3969/j.issn.1004-5619.2011.01.003

• 论著 • 上一篇    下一篇

猪眼玻璃体液钾离子、次黄嘌呤浓度变化与PMI推断

方  超1，王松才2，孙立敏2，张小婷2，龙伟清3，竞花兰1   

  1. （1. 中山大学 中山医学院法医病理教研室，广东 广州 510080； 2. 广州市刑事科学技术研究所，广东 广州 510030； 3. 中山大学 附属第一医院检验科，广东 广州 510080）
  • 发布日期:2011-02-25 出版日期:2011-02-28
  • 通讯作者: 通信作者：竞花兰，女，教授，硕士研究生导师，主要从事法医病理学、法医临床学研究；E-mail：jinghl@mail.sysu.edu.cn
  • 作者简介:方超（1984—），男，江苏扬州人，硕士研究生，主要从事法医病理学研究；E-mail：fch76@126.com

Concentration Changes of Potassium and Hypoxanthine in Vitreous Humor of Swine and Its Application to Postmortem Interval Estimation

FANG CHAO1, WANG SONG-CAI2, SUN LI-MIN2, ZHANG XIAO-TING2, LONG WEI-QING3, JING HUA-LAN1   

  1. （1. Department of Forensic Pathology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; 2. Guangzhou Criminal Science and Technology Institute, Guangzhou 510030, China; 3. Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China）
  • Online:2011-02-25 Published:2011-02-28

摘要： 目的 研究猪眼玻璃体液成分变化及其与死亡时间（postmortem interval，PMI）的关系。 方法 放血处死家猪后取带眼睑的眼球96只，随机分为24组，在避光、温度（15±2） ℃、湿度（50±5）%条件下分别放置2~96 h，采集玻璃体液，运用全自动生化检测仪、超高效液相色谱检测K+、Na+、Cl-及次黄嘌呤（hypoxanthine，Hx）浓度；用SPSS统计软件检测数据进行统计分析。 结果 玻璃体液中K+、Hx的浓度与PMI呈线性相关，R2分别为0.767和0.793；K+和Hx两者浓度与PMI经二元线性回归分析，R2为0.866；玻璃体液中Na+、Cl-浓度与PMI无明显相关性。 结论 玻璃体液K+、Hx浓度变化可作为较为客观的推断PMI的参考指标，两者与PMI的二元回归方程推断死亡时间有更高的准确性。

关键词: 法医病理学, 次黄嘌呤, 钾, 玻璃体液, 死亡时间, 猪

Abstract: Objective To investigate the relationship between postmortem interval（PMI） and concentration changes of components in swine vitreous humor. Methods Ninety-six porcine eyes from swine dying from acute massive hemorrhage, being randomly divided into 24 groups, were stored in dark situation, at temperature of （15±2） ℃ and humidity of（50±5）% for 2-96 hours separately. The vitreous humor was collected. Concentrations of K+, Na+, Cl- and hypoxanthine（Hx） were analyzed by automatic biochemical analyzer and ultra performance liquid chromatograph（UPLC）. The data were statistically analyzed by SPSS software. Results Linear regression analysis showed that concentrations of vitreous K+ and Hx were positively correlated with PMI（R2=0.767 and R2=0.793, respectively）. Binary linear regression showed a higher correlation for K+ and Hx with PMI estimation（R2=0.866）. PMI was not significantly correlated with vitreous Na+ and Cl- concentrations. Conclusion Vitreous K+ and Hx concentrations can be used as the objective markers for PMI estimation. The binary linear regression functions of vitreous K+ and Hx concentrations with PMI are more accurate for estimating the PMI.

Key words: forensic pathology, hypoxanthine, potassium, vitreous humor, postmortem interval, swine

中图分类号: