法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (1): 18-20.DOI: 10.3969/j.issn.1004-5619.2012.01.004

• 论著 • 上一篇    下一篇

急性心肌缺血猝死心肌中FAPα和TGF-β1的表达

袁翔天1，彭  雪2，靳占峰2   

  1. （1. 呼伦贝尔市公安局刑事科学技术支队，内蒙古 呼伦贝尔 021008； 2. 哈尔滨医科大学法医教研室，黑龙江 哈尔滨 150081）
  • 发布日期:2012-02-25 出版日期:2012-02-28
  • 通讯作者: 靳占峰，男，教授，硕士研究生导师，主要从事法医病理学、肿瘤间质及器官纤维化研究；E-mail：jinzf2003@yahoo.com.cn
  • 作者简介:袁翔天（1970—），男，内蒙古鄂伦春自治旗人，副主任法医师，主要从事法医病理死亡机制研究；E-mail：wjyxt@sina.com
  • 基金资助:

    黑龙江省政府博士后科研启动基金（LRB07-144）

Expression of FAPα and TGF-β1 in Sudden Deaths due to Acute Myocardial Ischemia

YUAN XIANG-TIAN1, PENG XUE2, JIN ZHAN-FENG2   

  1. （1. Detachment of Criminal Science and Technology, Public Security Bureau of Hulunbuir, Hulunbuir 021008, China; 2. Department of Forensic Medicine, Harbin Medical University, Harbin 150081, China）
  • Online:2012-02-25 Published:2012-02-28

摘要： 目的 探讨成纤维细胞激活蛋白α（fibroblasts activating protein α，FAPα）和转化生长因子β1（transforming growth factor-β1，TGF-β1）在急性心肌缺血猝死者心肌细胞胞浆中的表达。 方法 选取47例尸检案例心脏标本，按照死因分为对照组、急性心肌梗死组及复发性心肌梗死组，应用免疫组织化学技术检测心肌细胞胞浆中FAPα和TGF-β1的表达，染色结果经图像分析系统采集照片后，测定其阳性面积率及平均光密度值，比较各组间阳性信号的差别。 结果 急性心肌梗死组和复发性心肌梗死组的心肌缺血区心肌细胞胞浆中FAPα和TGF-β1呈强阳性表达，对照组心肌细胞胞浆中FAPα无表达，TGF-β1呈弱阳性表达。急性心肌梗死组和复发性心肌梗死组分别与对照组心肌细胞内FAPα和TGF-β1相比，差异有统计学意义（P<0.05）。 结论 FAPα和TGF-β1联合应用可作为急性心肌缺血猝死的诊断指标。

关键词: 法医病理学；免疫组织化学；心肌缺血；转化生长因子&beta, 1；成纤维细胞激活蛋白&alpha

Abstract: Objective To explore the expression of fibroblast activation protein α （FAPα） and transforming growth factor-β1 （TGF-β1） in myocardial cytoplasm for the cases of sudden death due to acute myocardial ischemia. Methods The heart tissues of 47 cases were collected. All cases were divided into three groups： control group, acute myocardial infarction group and recurrent myocardial infarction group. FAPα and TGF-β1 expressions were explored in myocardial cytoplasm by immunohistochemistry technology. The staining results were collected by image analysis system and then the positive area ratio and average optical density were detected. The positive signal differences were compared among the groups. Results Strong FAPα and TGF-β1 expressions were detected in myocardial cytoplasm in both acute and recurrent myocardial infarction groups. The expression of FAPα was not detected in myocardial cytoplasm in control group and TGF-β1 expression showed a weak positive result. FAPα and TGF-β1 expressions showed the statistical difference（P<0.05） in myocardial infarction （acute and recurrent） groups and control group. Conclusion FAPα and TGF-β1 can be the diagnostic markers for determing acute myocardial infarction.

Key words: forensic pathology, immunohistochemistry, myocardial ischemia, transforming growth factor-β1, fibroblast activation protein α

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