法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (4): 281-286.DOI: 10.3969/j.issn.1004-5619.2012.04.010

• 论著 • 上一篇    下一篇

应用16S rDNA短序列片段鉴定常见嗜尸性丽蝇

石  坚1，郭亚东1，匡栩源1，兰玲梅1，蔡继峰1，2，王红杰3   

  1. 1. 中南大学基础医学院法医学系，湖南 长沙 410013； 2. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063； 3. 中山市公安局小榄分局，广东 中山 528415
  • 发布日期:2012-08-25 出版日期:2012-08-28
  • 通讯作者: 蔡继峰，男，教授，主要从事法医昆虫学研究；E-mail：cjf_jifeng@163.com
  • 作者简介:石坚（1988—），男，陕西西安人，2008级临床医学学生；E-mail：shijian88116@163.com
  • 基金资助:

    湖南省自然科学基金资助项目（11JJ5075）；上海市法医学重点实验室资助项目（KF1203）

Identification of Sarcosaphagous Calliphorid Flies by Analyzing the Sequence of 16S rDNA

SHI JIAN1, GUO YA-DONG1, KUANG XU-YUAN1, LAN LING-MEI1, CAI JI-FENG1,2, WANG HONG-JIE3   

  1. 1. Department of Forensic Medicine, School of Basic Medical Science, Central South University, Changsha 410013, China; 2. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China; 3. Xiaolan Branch of Zhongshan Public Security Bureau, Zhongshan 528415, China
  • Online:2012-08-25 Published:2012-08-28

被引次数

10

摘要： 目的 利用16S rDNA中289 bp序列对常见嗜尸性丽蝇进行种类鉴定。 方法 从14个省市户外采集丽蝇科3属6种共计26个蝇类样本。利用CTAB法提取蝇类胸肌DNA，对16S rDNA的289 bp基因片段进行PCR扩增，检测扩增产物，测序并上传至GenBank，按邻接法构建系统发育树，通过序列分析建立种内及种间进化分歧表。 结果 上述蝇类按照不同属、种分别聚类，其种内进化分歧均数均为0，种间进化分歧均数在0.3%～6.5%。 结论 16S rDNA上289 bp基因序列能有效地对国内常见丽蝇进行种类鉴定，应用该片段具有快速、低耗等优点，为应用嗜尸性丽蝇推断死亡时间提供可能。

关键词: 法医遗传学；昆虫学；DNA, 核糖体；嗜尸性昆虫；丽蝇科

Abstract: Objective To explore the application of a 289 bp fragment of the 16S rDNA gene to identify various species of sarcosaphagous Calliphorid flies. Methods Twenty-six Calliphorid flies were collected from 14 Chinese provinces. All specimens were properly assigned into three genera and six species. The DNA of the pectoralis was extracted using CTAB method. Then PCR amplification was done for the 289 bp fragment of the 16S rDNA gene. The PCR products were then purified and sequenced, and the obtained sequences were uploaded to GenBank. The phylogenetic tree was built by the neighbor-joining method and intraspecific and interspecific divergences were calculated by sequence analysis. Results The above 26 sarcosaphagous flies could be well clustered according to different genera and species. The evolutional intraspecific values were all zero, the evolutional interspecific variations varied from 0.3% to 6.5%. Conclusion The 289 bp fragment of the 16S rDNA of sarcosaphagous flies can be effectively used to identify most of the flies at species level. This method appears to be fast and low dissipative, which might be used to estimate postmortem interval by sarcosaphagous flies.

Key words: forensic genetics, entomology, DNA, ribosomal, sarcosaphagous insects, Calliphoridae

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