法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (5): 336-339.DOI: 10.3969/j.issn.1004-5619.2010.05.005

• 论著 • 上一篇    下一篇

常见嗜尸性甲虫及蝇类虫体不同部位mtDNA的提取效果

郭亚东1，蔡继峰1，2，苏日娜1，常云峰1，兰玲梅1，李  响1，文继舫1   

  1. （1. 中南大学 基础医学院法医学系，湖南 长沙 410013； 2. 中南大学 湘雅二医院心理中心，湖南 长沙 410011）
  • 发布日期:2010-10-25 出版日期:2010-10-28
  • 通讯作者: 通信作者：蔡继峰，男，副教授，主要从事法医昆虫学研究；E－mail：cjf_jifeng@163.com
  • 作者简介:郭亚东（1982—），男，河南郑州人，博士，主要从事法医病理学方面的研究；E－mail：gdy82@126.com
  • 基金资助:

    国家自然科学基金资助项目（30672354）；2007年度湖南省优秀青年骨干教师培养项目（湘教通[2007]214）

Comparison of mtDNA Extraction from Different Parts of Sarcosaphagous Insects

GUO YA-DONG1, CAI JI-FENG1,2, SU RI-NA1, CHANG YUN-FENG1, LAN LING-MEI1, LI XIANG1, WEN JI-FANG1   

  1. （1. Department of Forensic Medicine, School of Basic Medical Science, Central South University, Changsha 410013, China; 2. Center of Psychology, Second Xiang-Ya Hospital, Central South University, Changsha 410011, China）
  • Online:2010-10-25 Published:2010-10-28

被引次数

20

摘要： 目的 探讨改良后十六烷基三甲基溴化铵（cetyltriethylammnonium bromide，CTAB）法对常见嗜尸性昆虫虫体不同部位mtDNA的提取效果。 方法 随机采集放置在呼和浩特地区室外草地家兔尸体上丽蝇科的丝光绿蝇［Lucilia sericata（Meigen）］、埋葬甲科的Nicrophorus fossor（Erichson）各13例，采用改良CTAB法分别提取每只昆虫头部、胸肌、腿部、翅膀4个部位的mtDNA，核酸蛋白测定仪检测DNA纯度及质量浓度，琼脂糖凝胶电泳检测其PCR产物，测定PCR产物序列，上传GenBank。 结果 13例丝光绿蝇［Lucilia sericata（Meigen）］的胸肌均提取到了DNA，10例头部样本、6例腿部样本、4例翅膀样本得到DNA；13例Nicrophorus fossor（Erichson）胸肌DNA均提取成功，5例头部样本、8例腿部样本及3例翅膀样本提取出DNA。 结论 改良CTAB法可用于对嗜尸性昆虫胸肌及其他部位进行DNA的提取。

关键词: 法医遗传学, 昆虫学, DNA, 线粒体, 嗜尸性昆虫

Abstract: Objective To explore mitochondrial DNA （mtDNA） extraction effects of different parts from sarcosaphagous insects using improved cetyltriethylammnonium bromide（CTAB） method. Methods Thirteen Lucilia sericata（Meigen） and 13 Nicrophorus fossor（Erichson） were collected from the corpses of rabbits placed on the outdoor lawn in Huhehot district. Four parts （head, chest muscle, legs and wings） of insect were collected, and the mtDNA of all samples were extracted using CTAB method. The purity and concentration were tested using protein and nucleic acid spectrophotometry. The integrity of the extracted mtDNA and PCR products were checked by agarose gel electrophoresis. The PCR products were sequenced and the obtained sequences were imputed into GenBank for comparison. Results mtDNA were successfully extracted from 10 head samples, 6 legs samples, 4 wing samples and 13 chest muscle samples of the Lucilia sericata（Meigen）. Also, mtDNA were successfully extracted from 5 head samples, 8 legs samples, 3 wing samples and 13 chest muscle samples of the Nicrophorus fossor（Erichson）. Conclusion mtDNA can be obtained from chest muscle and other parts of sarcosaphagous insects using the improved CTAB method.

Key words: forensic genetics, entomology, DNA, mitochondrial, sarcosaphagous insects

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