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法医学杂志 2018, Vol. 34 Issue (3) :264-269    DOI: 10.12116/j.issn.1004-5619.2018.03.010
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19个STR基因座在山东三个文化区人群中的遗传多态性
张珊珊1,2,唐玉秋2,张茂修2,3,高洪梅2,3,王  昌2,3,汪运山2,3,韩淑毅2
1. 山东大学齐鲁医学院,山东 济南 250012; 2. 山东大学附属济南市中心医院,山东 济南 250013; 3. 济南迪恩法医司法鉴定所,山东 济南 250013
Genetic Polymorphisms of 19 STR Loci in Populations of Three Culture Region in Shandong
ZHANG Shan-shan1,2, TANG Yu-qiu2, ZHANG Mao-xiu2,3, GAO Hong-mei2,3, WANG Chang2,3, WANG Yun-shan2,3, HAN Shu-yi2
1. Cheeloo College of Medicine, Shandong University, Jinan 250012, China; 2. Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China; 3. Jinan Dien Forensic Judical Appraisal Institute, Jinan 250013, China

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摘要 目的 分析19个常染色体STR基因座在山东鲁东、鲁中-鲁西北及鲁西南-鲁南文化区汉族人群中的遗传多态性,并探讨三个文化区人群之间的群体遗传学关系。 方法 应用Goldeneye?誖DNA身份鉴定系统20A试剂盒扩增山东三个文化区1 044名汉族无关个体的STR基因座,Modified-Powerstates软件统计19个常染色体STR基因座的等位基因频率和群体遗传学参数,Arlequin v3.5软件计算三个文化区人群之间的遗传距离,MEGA v4.0软件绘制系统发育树。 结果 19个常染色体STR基因座中,有15个在山东三个文化区人群中的杂合度(heterozygosity,H)大于0.7,多态信息含量(polymorphic information content,PIC)大于0.7,个体识别率(discrimination power,DP)大于0.9。三个文化区人群中,鲁中-鲁西北与鲁西南-鲁南人群之间的遗传距离最近(Fst=0.000 16),其次是鲁东与鲁西南-鲁南人群(Fst=0.000 36),鲁东与鲁中-鲁西北人群之间的遗传距离最远(Fst=0.000 66,P<0.05)。 结论 19个常染色体STR基因座在山东三个文化区汉族人群中具有较好的多态性,其中15个STR基因座具有高度多态性,且鲁东与鲁中-鲁西北人群之间存在遗传学差异。
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张珊珊
唐玉秋
张茂修
关键词法医遗传学   多态现象   遗传   短串联重复序列   汉族   山东     
Abstract: Objective To analyse the genetic polymorphisms of 19 autosomal STR loci in Han population of east, middle-northwest and southwest-south Shandong and to explore its genetic relationships among the population of these three regions. Methods STR loci of 1 044 unrelated Han individuals in three Shandong regions were typed with a Goldeneye?誖DNA ID System 20A kit. The allele frequency and population genetics parameters of 19 autosomal STR loci were statistically analysed by Modified-Powerstates software. The genetic distances among the population in three regions were calculated by Arlequin v3.5 software. The phylogenetic tree was conducted using MEGA v4.0 software. Results Fifteen of 19 autosomal STR loci were detected with the H values greater than 0.7, PIC values greater than 0.7, and DP values greater than 0.9 in the populations of all three Shandong regions. Among the populations in these three regions, the genetic distance between the populations in middle-northwest and southwest-south Shandong was closest (Fst=0.000 16), followed by east and southwest-south Shandong (Fst=0.0003 6). The genetic distance between the populations in east and middle-northwest Shandong was the farthest (Fst=0.000 66, P<0.05). Conclusion The 19 autosomal STR loci show good genetic polymorphisms in Han population of three Shandong regions, and 15 of them are high. There are genetic differences between the populations in east and middle-northwest Shandong.
Keywordsforensic genetics   polymorphism, genetic   short tandem repeat   Han population   Shandong     
Fund:济南市科技局发展计划资助项目(066007);济南市卫生局资助项目(2006-09)
Corresponding Authors: 韩淑毅,女,博士,主管技师,主要从事分子遗传学和法医遗传学研究;E-mail:hanshuyi2007@126.com   
About author: 张珊珊(1991—),女,硕士研究生,主要从事法医遗传学研究;E-mail:804538642@qq.com
引用本文:   
张珊珊,唐玉秋,张茂修,等.19个STR基因座在山东三个文化区人群中的遗传多态性[J]  法医学杂志, 2018,34(3): 264-269
ZHANG Shan-shan, TANG Yu-qiu, ZHANG Mao-xiu,et al.Genetic Polymorphisms of 19 STR Loci in Populations of Three Culture Region in Shandong[J]  法医学杂志, 2018,34(3): 264-269
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