法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (6): 706-709.DOI: 10.12116/j.issn.1004-5619.2019.06.011

• 技术与应用 • 上一篇    下一篇

肺组织硅藻定量分析在实验动物溺死诊断中的应用

赵建1，2， 罗银洲3， 王玉仲4， 肖成5， 石河2， 胡孙林2， 康晓东2， 徐曲毅2， 成建定1， 刘超1，2   

  1. 1. 中山大学中山医学院法医学系，广东 广州 510080； 2. 广州市刑事科学技术研究所 法医病理学公安部重点实验室，广东 广州 510030； 3. 十堰市公安局，湖北 十堰 442000； 4. 广东江门中医药职业技术学院，广东 江门 529000； 5. 南方医科大学法医学院，广东 广州 510515
  • 发布日期:2019-12-25 出版日期:2019-12-28
  • 通讯作者: 成建定，男，博士，教授，主任法医师，博导，主要从事法医病理学研究；E-mail：chengjd@mail.sysu.edu.cn 刘超，男，博士，教授，主任法医师，博导，主要从事个体识别及死因鉴定研究；E-mail：liuchaogzf@163.com
  • 作者简介:赵建（1988—），男，主要从事法医病理学检验鉴定及法医学硅藻检验研究；E-mail：zhaojian0721@163.com
  • 基金资助:
    国家自然科学基金资助项目（81430046）；公安部技术研究计划资助项目（2015JSYJA03）；广东省科技计划资助项目（2015A020217001）；公安部科技强警基础工作专项资助项目（2017GABJC07）

Application of Quantitative Analysis of Diatoms in Lung Tissue for the Diagnosis of Drowning of Experimental Animals

ZHAO Jian1,2, LUO Yin-zhou3, WANG Yu-zhong4, XIAO Cheng5, SHI He2, HU Sun-lin2, KANG Xiao-dong2, XU Qu-yi2, CHENG Jian-ding1, LIU Chao1，2   

  1. 1. Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; 2. Key Laboratory of Forensic Pathology, Institute of Guangzhou Forensic Science, Ministry of Public Security, PRC, Guangzhou 510030, China; 3. Shiyan Public Security Bureau, Shiyan 442000, Hubei Province, China; 4. Guangdong Jiangmen Chinese Medical College, Jiangmen 529000, Guangdong Province, China; 5. School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China
  • Online:2019-12-25 Published:2019-12-28

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摘要： 目的 探讨肺组织硅藻检验在法医学溺死诊断中的应用价值。 方法 将实验动物随机分为溺死组、死后入水组和陆地死亡组，采用微波消解-真空抽滤-扫描电镜联用的硅藻检验方法对肺组织和溺液硅藻进行定量分析，记录肺组织与溺液硅藻含量的比值（ratio of content of diatoms in lung tissue and drowning fluid，CL/CD）。 结果 溺死组实验兔的CL/CD值（5.82±3.50）高于死后入水组（0.47±0.35）；溺死组实验猪肺叶不同部位的CL/CD值均高于死后入水组；在海水、咸淡水交界、河道淡水及湖泊淡水中，溺死组实验猪的CL/CD值均高于死后入水组（P<0.05）。动物实验中，CL/CD值>1.6的案例均来自溺死组。 结论 CL/CD值是溺死诊断中有较好应用前景的指标。

关键词: 法医病理学；溺水；硅藻类；动物, 实验；兔；猪；肺

Abstract: Objective To discuss the application value of diatom examination in lung tissue for the forensic diagnosis of drowning. Methods The experimental animals were divided randomly into drowning, postmortem submergence and dying on land group. Diatoms in lung tissue and drowning fluid were analyzed quantitatively by microwave digestion-vacuum filtration-automated scanning electron microscopy diatom examination method. The ratios of content of diatoms in lung tissue and drowning fluid （CL/CD ratio） were recorded. Results The CL/CD ratios of experimental rabbits in the drowning group （5.82±3.50） were much higher than that of postmortem submergence group （0.47±0.35）; the CL/CD ratios of different parts of the lung lobes of experimental pigs in the drowning group were higher than that of postmortem submergence group （P<0.05）; in seawater, brackish water, river fresh water and lake fresh water, the CL/CD ratios of experimental pigs in the drowning group were higher than that of postmortem submergence group （P<0.05）. In animal experiments, all the cases with CL/CD ratio >1.6 were from drowning group. Conclusion CL/CD ratio is an indicator with good application prospects in the diagnosis of drowning.

Key words: forensic pathology, drowning, diatoms, animals, laboratory, rabbits, swine, lung