›› 1993, Vol. 0 ›› Issue (2): 51-53+2.

• 论文 • Previous Articles     Next Articles

Simultaneous Isoelectric Focusing of EsD EAP ADA on Uitrathin-layer Polyacrylamide Gels.

LI LI INSTITUTE OF FORENSIC SCIENCES,MINISTRY OF JUSTICE,PRC;SHANGHAI 200063   

  • Online:1993-04-25 Published:1993-04-28

Abstract: A nonequilibrium ultrathin-layer polyacrylamide gel isoelectric focusing(ULPAGIEF)method incorporating the chemical spacer HEPES was developed for simultaneous typing of Esterase D(EsD),Red Cell Acid Phosphatase(EAP)and Adenosine Deaminase (ADA)isoenzyme systems.UIPAG contained 4%(v/v)PH4-6.5 Ampholine and 3%(w/v) HEPES.This approach had high reso- lution capacity and great discriminating power of genetic markers.