Identification of Death Caused by Snake Biting-Establishment of ABC-ELISA for the Detection of Krait Venom

›› 1995, Vol. 0 ›› Issue (2): 0-0.

Identification of Death Caused by Snake Biting-Establishment of ABC-ELISA for the Detection of Krait Venom

YAN ZUKANG(FACLUTY OF FORENSIC MEDICINE, SHANGHAI MEDICALUNIVERSITY; SHANGHAI 200032), ZHU JIAZHEN (FACULTY OF FORENSIC MEDICINE, SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES:GUANGZHOU510089)

Online:1995-04-25 Published:1995-04-28

Abstract

Abstract: A double antibody ABC- ELISA using NHBH conjugatehas ben investigated on krait venom standards, which revealed that the optimal reaction over the range was 0.25- 250 ng/ml, intra- and inter- assay variations were lessthan 8% and 16%, recovery was 78% -89% within thesame range, the cross Teaction with cobra venom was negligible. The studies of the factors affectins detection of the venom through three orthogonal tables show that the serumand kidney tissue from the experimental animals were the most satisfaCtory samples for identifying snake biting within 720 hours after death. The results of the experiment data indicate a strong reliability for routine test.

Key words: snake-bite, venom, ELISA

YAN ZUKANG(FACLUTY OF FORENSIC MEDICINE;SHANGHAI MEDICALUNIVERSITY;SHANGHAI 000);ZHU JIAZHEN (FACULTY OF FORENSIC MEDICINE;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES:GUANGZHOU00). Identification of Death Caused by Snake Biting-Establishment of ABC-ELISA for the Detection of Krait Venom[J]. , 1995, 0(2): 0-0.

[1]	SUN LAI-JING;XU GE-LIN;WANG HUA-LIN;WU JIE;HU ZHI-HONG;ZHOU YI-WU (.DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;.STATE KEY LABORATORY OF VIROLOGY;WUHAN INSTIT. Application of Immunofluorescence and Sandwich ELISA with Double-antibodies in Detection of Human Rabies [J]. , 2007, 0(6): 411-413+.
[2]	WANG HUI-JUN;DING YUN-CHUAN (DEPARTMENT OF PATHOLOGY;THE FIRST MILITARY MEDICAL UNIVERSITY;GUANGZHOU 0). A analysis of ELISA on the time-related expression of IL-2 and TNF-a during the healing process of wound in rat skin [J]. , 2003, 0(1): 10-12.
[3]	SHEN YI-WEN;LU CHAO;ZHAO ZI-QIN;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL SCHOOL;FUDAN UNIVERSITY;SHANGHAI000). Tryptase and fatal anaphylaxic reaction [J]. , 2002, 0(3): 132-134+.
[4]	BAO LEI;ET AL (LIAONING BASIC MEDICAL INSTITUTE;SHENYANG 000;PRC). The MN typing of whole blood and bloodstains by the method of one-step sandwich ELISA [J]. , 2000, 0(3): 146-147+.
[5]	SHEN YIWEN;ET AL( DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL UNIVERSITY;SHANGHAI 000;PRC). The effect of injuries on Fn synthesized by FBs and its implication in wound timing [J]. , 2000, 0(3): 129-131+.
[6]	YU XIAOJUN;CHEN GUODI;GOU QING;ET AL(DEPARTMENT OF FORENSIC MEDICINE;JILIN MEDICAL COLLEGE;JILIN 00;P.R.CHINA). MBP content in serum after closed diffuse cranial injury in rats [J]. , 1999, 0(2): 0-0.
[7]	ZHOU DAPENG ET AL (DEPARTMENT OF BIOCHEMISTRY;SHANGHAI MEDICAL UNIVERSITY;SHANGHAI000). A New Method for Preparation of Enzyme-Antibody Conjugate and Its Application in Serology [J]. , 1995, 0(2): 0-0.
[8]	ZHOU BIN;GUO JINGYUAN DEPARTMENT OF FORENSIC SEROLOGY SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES. Differentiation of Group ABSe Body Fluids (or Stains)from Mixture of Group ASe and BSe Body Fluids(or Stains)by ELISA [J]. , 1990, 0(2): 21-26+4.

Identification of Death Caused by Snake Biting-Establishment of ABC-ELISA for the Detection of Krait Venom

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