Abstract: A modified periodate oxidation method was developed to label rabbit-ant-human IgG with HRP.The antibody was first hydrazized with adipic dihydrazide,then the hydrazide groups of the antibody-hydrides reacted with the aldehydes that were formed on the sugars of HRP by periodate oxidation. The labeled antibody was utilized for species identification by dot- ELISA through detection of human immunoglobin and proved to be specialized and sensitive.

Key words: enzyme-antibody conjugates, periodate oxidation, blood stains, dot-ELISA