Abstract: N-hydroxysuccinimido 6-(biotinylamido) hexanoate (NHBH) prepared from d-biotin was measured with mass spectroscopy, which gave a molecular weight at 454. Further characterization by infraied spectroscopy confirmed the desired functional groups. The optimization of the molar incorporation ratios between NHBH and horse anti-Bungarus IgG (ab') 2 previously purified by affinity Chromatography is investigated. The enhancement of the dilution titer of the conjugate was 4 and 8 fold higher than that of conven tiotal biotin-theied conjugate and peroxidase conjugate, respectively.

Key words: N-hydroxysuccinimido 6-(biotinlamido) hexanoate, bungarotoxin, d-biotin, snake biting