Immunohistochemical Study on Vital Reaction of Ligature Mark in Rats

Abstract

Abstract: The model of ligature mark injuries (5 min to 1 hr.) were produced on the neck of 40 wistar rats. The positive staining and distribution of Fibronectin (Fn) and Fibrinogen (Fb) in routine paraffin sections of the region of ligature mark were observed using streptuvidin-biotinperoxi-dase complex (SABC) technique. The results showed that a positive reaction of Fn and Fb could be detected in the skin corium under ligature marks only 5 minutes after injury. The longer the injury-time, the stronger positive staining could be observed.The strongest band-sharped positive stining of ligature marks where the epiderm was lost. No positive reaction could be observed in control groups with the exception of a few postmortem ligature marks which a weak akining of Fb in the skin corium could be found. The results indicate that detecting Fn and Fb are useful for distinguishing between atemortem and postmortem ligature mark.

Key words: Ligature mark, Vital reaction, Fibronectin, Fibrinogen, Immunohistochemical

CHEN XIAORUT;QING QISHENG;ZHANG YIHU;ZHU SHAOHUA AND ZHAO XIAOHONG (DEPARTMENT OF FORENSIC MEDICINE;TONG JI MEDICAL UMVERSITY;HUBEI WUHAN O00). Immunohistochemical Study on Vital Reaction of Ligature Mark in Rats[J]. , 1996, 0(3): 0-0.

[1]	LIU Ran, GE Lu-zou, ZHANG Hai-dong，et al.. Expressions of TGF-β1 and EⅢA-FN after Rat Skeletal Muscle Contusion and Wound Age Estimation [J]. 法医学杂志, 2019, 35(2): 154-159.
[2]	TAO LI, MA JIAN-LONG, CHEN LONG. Research Progress on Estimation of Early Postmortem Interval [J]. , 2016, 32(6): 444-447.
[3]	HE MENG, ZHAO HAI, SHEN YI-WEN, ET AL.. Expression of EIIIA+ Fibronectin in Incised Wound of Rat’s Skin [J]. , 2014, 30(6): 409-412.
[4]	GAO SHU-HONG;LIU CONG;TAN QIU-FENG;HUANG FEI-JUN (. WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY CHENDU 00;CHINA;.CENTER OF INFECTIONS DISEASES;WEST CHINA HOSPITAL;SICHUAN UNIVERSITY CHENGDU 00;CHINA). Expression of Myocardial CollagenⅠin Early Acute Myocardial Ischemic of Human [J]. , 2008, 0(3): 175-177+.
[5]	YU SONG;XUE AI-MIN;HE MENG;ZHAO ZI-QIN;SHEN YI-WEN;GU YUN-JU(.HUZHOU PUBLIC SECURITY BUREAU;HUZHOU 000;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA). The Relationship Between Fibronectin EDA/EDB mRNA in Skin Fibroblast and Injury Time [J]. , 2007, 0(3): 188-190.
[6]	ZHANG JIAN-HUA;LIU NING-GUO;CHEN YI-JIU;HUANG XIAO-HUA(INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA). Validation Studies of the Test Strip of Colloidal Gold-Labeled Anti-Fibronectin EIIIA Splicing Variant for Estimating the Time of Injury [J]. , 2006, 0(3): 171-172+.
[7]	LIU NING-GUO;CHEN YI-JIU;LU REN-QUAN;HUANG XIAO-HUA;ZHANG JIAN-HUA (. INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;CHINA;.SHANGHAI METERIA MEDICA BIOENGIERING INSTITUTE;SHANGHAI 0000;CHINA). Preliminary Application on Test Paper of Gold-labelled Antibody against Fibronectin EⅢA Splicing Variant [J]. , 2006, 0(2): 81-83.
[8]	LI WEN-SHENG;GONG QING-JIN;LU LONG-WU;ZHANG YI-JUN;CHENG JIAN-DING (.THE BUREAU OF PUBLIC SECURITY OF GONGBEI;ZHUHAI 00;CHINA;. DEPARTMENT OF FORENSIC PATHOLOGY;BASIS MEDICAL COLLEGE;SUN YAT-SEN UNIVERSITY;GUANGZHOU 000;CHINA). Fibronectin immunohistochemical Staining for Diagnosing Sudden Death Caused by Viral Myocarditis [J]. , 2006, 0(2): 84-85+1.
[9]	YANG JING;WANG YE;CHEN XIAO-GANG;PENG QI-YI;LI LEI-BO;LIU MIN (.WEST CHINA COLLEGE OF PRECLINICAL MEDICINE AND FORENSIC SCIENCES OF SICHUAN UNIVERSITY;CHENDU 00;CHINA;. JINAN PUBLIC SECURITY BUREAU;JINAN 00;CHINA). Expression of Caspase-8 after Brain Injury from Fluid Percussion in Rats [J]. , 2006, 0(1): 1-3+99.
[10]	XUE AI-MIN;ZHAO ZI-QIN(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL SCHOOL;FUDAN UNIVERSITY;SHANGHAI000;CHINA). The research progression of fibronectin EDA's expression and functions [J]. , 2003, 0(3): 190-193.
[11]	XUE AI-MIN;ZHAO ZI-QIN;SHEN YI-WEN;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL SCHOOL;FUDAN UNIVERSITY;SHANGHAI000 ;CHINA). The relationship between expression of FN in human skin injury and early injury time [J]. , 2003, 0(3): 140-142.
[12]	XUE AI-MIN;WANG HUA;YE RONG;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL SCHOOL;FUDAN UNIVERSITY;SHANGHAI000). Molecular cloning of recombinant fibronectin EDA and EDB fusion protein [J]. , 2002, 0(3): 140-143.
[13]	LIU NING-GUO;CHEN YI-JIU;HUANG XIAO-HUA;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI000). Expression of EⅢA-fibronectin in injured rat skin used in estimation of wound interval [J]. , 2002, 0(3): 129-131.
[14]	CHEN YI-JIU;LIU NING-GUO;HUANG XIAO-HUA;ET AL (THE INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;SHANGHAI 000). In vivo distribution and expression after skin injury of fibronectin splicing variants [J]. , 2001, 0(4): 193-195.
[15]	CHEN LONG;ET AL.( DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000). Study on the expression of fibronectin after cerebral contusion in rats for timing of injuries [J]. , 2001, 0(1): 1-3+61.

Immunohistochemical Study on Vital Reaction of Ligature Mark in Rats

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics