Influence of Meliorated AS-PCR Primer for ABO Genotyping

›› 2006, Vol. 0 ›› Issue (4): 275-276.

Influence of Meliorated AS-PCR Primer for ABO Genotyping

WANG XIN-JIE1, DIAO LI-JIANG1, FENG JIAN-ZHONG1, LU GUI-PING2 (1.WEIFANG PUBLIC SECURITY BUREAU, WEIFANG 261061,CHINA; 2.DEPARTMEMT OF PUBLIC SECURITY OF SHANDONG,JINAN 250001,CHINA))

Online:2006-08-25 Published:2006-08-28

Abstract

Abstract: Objective To improve the correct rate of ABO genotyping by meliorating AS-PCR primer. Methods The primerP1 was changed into primerP1′ by substituting the fifth base G for C of 3′end and the ABO genotyping results of primer P1 and P1′ was compared and analysed. Results The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer. Conclusion The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1′.

Key words: AS-PCR, meliorated primer, 3′-end, base, ABO genotyping

WANG XIN-JIE;DIAO LI-JIANG;FENG JIAN-ZHONG;LU GUI-PING (.WEIFANG PUBLIC SECURITY BUREAU;WEIFANG 0;CHINA;.DEPARTMEMT OF PUBLIC SECURITY OF SHANDONG;JINAN 000;CHINA)). Influence of Meliorated AS-PCR Primer for ABO Genotyping[J]. , 2006, 0(4): 275-276.

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Influence of Meliorated AS-PCR Primer for ABO Genotyping

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