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Postmortem Interval Estimation WANG Zhen-yuan

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Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select 法医学杂志    2018, 34 (5): 457-458.   DOI: 10.12116/j.issn.1004-5619.2018.05.001 Abstract （303）      PDF（pc） （7039KB）（957）       Knowledge map    Save Related Articles | Metrics | Comments（0） Select Current Research and Prospects on Postmortem Interval Estimation WANG Qi, LIN Han-cheng, XU Ji-ru,et al. 法医学杂志    2018, 34 (5): 459-467.   DOI: 10.12116/j.issn.1004-5619.2018.05.002 Abstract （1631）      PDF（pc） （7039KB）（2483）       Knowledge map    Save The researches on postmortem interval （PMI） estimation are very important and meaningful in forensic science. PMI estimation is also an important issue that must be solved in practice of forensic pathology. There are many defects existing in traditional methods for PMI estimation, so it is imperative to introduce new pathways. With the emergence of various new technologies, the researches on PMI estimation have a tendency from simple to complex with a growth of data. The present review firstly summarizes a series of methods used for PMI estimation, and then gives an outlook for the application of artificial intelligence algorithms in this field. Related Articles | Metrics | Comments（0） Select Standardized Application of Forensic Entomology and Application Data of Sarcosaprophagous Insects in China WANG Yu, WANG Jiang-feng 法医学杂志    2018, 34 (5): 468-474.   DOI: 10.12116/j.issn.1004-5619.2018.05.003 Abstract （469）      PDF（pc） （973KB）（1293）       Knowledge map    Save Postmortem interval （PMI） estimation is a major problem in forensic medicine and a vital indicator of crime investigations. Forensic entomology provides a new way to the PMI estimation, and its superiority has been confirmed through many cases, especially in relation to the cadaver at the stage of advanced decomposition even skeleton. The technologies of forensic entomology have become more mature. This review describes the mechanism of forensic entomology used for PMI estimation, emphasizes the standardized application of technology, and lists the data for the reference of forensic experts in China. Related Articles | Metrics | Comments（0） Select Application of High-throughput Sequencing in Researches of Cadaveric Microorganisms and Postmortem Interval Estimation FU Xiao-liang, GUO Juan-juan, LIU Zhuo-ying，et al. 法医学杂志    2018, 34 (5): 475-481.   DOI: 10.12116/j.issn.1004-5619.2018.05.004 Abstract （588）      PDF（pc） （7038KB）（1360）       Knowledge map    Save Necrobiome is the main factor causing the cadaver decomposition. Studying the microbial succession during decomposition is one of the main tasks of forensic microbiology. The interactive relationships among cadaver, environment and microorganisms are complicated. The microbial succession study relies on macroscopic monitoring of community composition and the diversity change in each decomposition stage. With the maturity and development of high-throughput sequencing （HTS）, the structure and diversity of microbial communities in different environments have been successively revealed. A new breakthrough to explore the cadaveric microorganisms has been opened as well. It has become the research hotspots in forensic microbiology to reveal the microbial succession in the process of cadaver decomposition and to interpret the essence of various decomposition phenomena by using HTS, which can provide a new reference for postmortem interval （PMI） estimation. The present paper reviews studies on PMI estimation by using cadaveric microorganism. Problems and application prospects of forensic microbiology studies are discussed on the basis of the current application of HTS technology in the exploration of microbial succession. Related Articles | Metrics | Comments（0） Select Succession Law of Intestinal Flora after Death in SD Rats LI Huan, LIU Rui-na, ZHANG Si-ruo, et al. 法医学杂志    2018, 34 (5): 482-486.   DOI: 10.12116/j.issn.1004-5619.2018.05.005 Abstract （412）      PDF（pc） （7039KB）（1102）       Knowledge map    Save Objective To initially explore the sequential changes in the intestinal flora of corpse for the estimation of postmortem interval （PMI）. Methods Rats were sacrificed by cervical dislocation, and samples were taken from their intestines using cotton swab to extract the DNA of intestinal flora. The 16S rRNA V3 universal primers were selected for PCR, and the PCR products were used for denatured gradient gel electrophoresis. The diversity and similarity analysis of intestinal flora were analyzed between groups, and the bands were cut from denaturing gradient gel electrophoresis. After purification, PCR and sequencing, the percentage of major bacteria in each group was obtained. Results The flora diversity showed a reduced tendency from 1st to 30th day after death （P<0.05）, while the intra-group similarity showed a downward trend （P<0.05）. The number of bands and intra-group similarity coefficient （Cs） on the first day was higher than that of other groups （P<0.05）. The intra-group Cs of the 25th and 30th day had a significant difference compared with the 5th day （P<0.05）. At the genus level, the intestinal flora was mainly composed of Enterococcus sp. on the 1th and 5th day after death, Bacillus thuringienssis was the dominant species on the 10th, 15th and 20th day, and Enterococcus faecalis became the dominant species on the 25th and 30th day. Conclusion The composition and structure of intestinal flora change significantly in rats with the time after death, which indicates that the succession of intestinal flora is related to the postmortem interval. Related Articles | Metrics | Comments（0） Select Homogeneity of Different Functional mRNA Indicators for Wound Age Estimation DU Qiu-xiang, ZHU Xi-yan, DONG Ta-na，et al. 法医学杂志    2018, 34 (5): 487-491.   DOI: 10.12116/j.issn.1004-5619.2018.05.006 Abstract （333）      PDF（pc） （7039KB）（870）       Knowledge map    Save Objective To explore the homogeneity level of four different functional mRNA （PUM2, TAB2, Cx45 and CHRNA1） expressions in rats with skeletal muscle contusion. Methods The relative expressions of PUM2, TAB2, Cx45 and CHRNA1 mRNAs were detected by quantitative real-time reverse transcription-polymerase chain reaction （RT-qPCR）. The coefficient of variation （CV） of the relative expressions for different individuals in each injury group was calculated. The extreme value of CV, cumulative variability, and CVCV were compared. Results A high CV of PUM2 and TAB2 mRNAs appeared on several different time points. However, the CV of Cx45 and CHRNA1 mRNAs was relatively low. The cumulative variability from high to low was PUM2, CHRNA1, TAB2 and Cx45 mRNAs. The relative expression of PUM2 mRNA was significantly higher than that of TAB2, Cx45 and CHRNA1 mRNAs （P<0.05）. There was no statistical significance （P>0.05） in the CVCV of the relative expression of TAB2, CHRNA1 and Cx45 mRNAs. Conclusion As the mRNAs involving in biological process regulation, PUM2 and CHRNA1 mRNAs show a lowest individual homogeneity of the relative expression followed by TAB2 mRNA. As the mRNAs participating in the composition of cellular structure, Cx45 and CHRNA1 mRNAs show a high individual homogeneity of the relative expressions. The functional classification should be considered for the screening of the mRNA indicators used for wound age estimation. Related Articles | Metrics | Comments（0） Select Relationship between Early Postmortem Interval and Electrical Conductivity of Rat Liver, Spleen and Kidney after Death in Rats ZHENG Zhe, ZHAI Xian-dun, XIA Zhi-yuan, et al. 法医学杂志    2018, 34 (5): 492-494.   DOI: 10.12116/j.issn.1004-5619.2018.05.007 Abstract （359）      PDF（pc） （7039KB）（800）       Knowledge map    Save Objective To determine the electrical conductivity （EC） of the liver, spleen and kidney of rats at different postmortem intervals （PMIs） within 24 hours for investigating the relationship between EC of different organs and early PMI. Methods Totally 45 SD rats were sacrificed by cervical dislocation and kept at a constant temperature of 25 ℃. Tissues were taken from the liver, spleen, and kidney of rats at 0, 3, 6, 9, 12, 15, 18, 21 and 24 h. Impregnating solution with a mass concentration 0.1 g/mL was prepared using deionized water. The EC value of impregnating solution with different organs was separately determined. The regression equations of EC and PMI for different organs were established, respectively. The relationship between EC of different organs and early PMI was analysed in deceased rats. Results The relationship between PMI and EC of the liver and spleen was well fitted with the linear equation. The liver showed the best fitting degree followed by the spleen, while the EC of the kidney showed no significant changes within 24 h. There was a good linear relationship between early PMI and the EC of the liver and spleen. Conclusion A good linear relationship between early PMI and the EC of the liver and spleen can be found in rats after death, which can be used for the early PMI estimation. Related Articles | Metrics | Comments（0） Select 法医学杂志    2018, 34 (5): 495-496.   DOI: 10.12116/j.issn.1004-5619.2018.05.008 Abstract （242）      PDF（pc） （7039KB）（727）       Knowledge map    Save Related Articles | Metrics | Comments（0） Select 法医学杂志    2018, 34 (5): 497-498.   DOI: 10.12116/j.issn.1004-5619.2018.05.009 Abstract （203）      PDF（pc） （7039KB）（674）       Knowledge map    Save Related Articles | Metrics | Comments（0） Select 法医学杂志    2018, 34 (5): 498-499.   DOI: 10.12116/j.issn.1004-5619.2018.05.010 Abstract （182）      PDF（pc） （7039KB）（668）       Knowledge map    Save Related Articles | Metrics | Comments（0） Select 法医学杂志    2018, 34 (5): 500-501.   DOI: 10.12116/j.issn.1004-5619.2018.05.011 Abstract （199）      PDF（pc） （7039KB）（648）       Knowledge map    Save Related Articles | Metrics | Comments（0） Select 法医学杂志    2018, 34 (5): 502-503.   DOI: 10.12116/j.issn.1004-5619.2018.05.012 Abstract （295）      PDF（pc） （7039KB）（904）       Knowledge map    Save Related Articles | Metrics | Comments（0）