法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (5): 567-571.DOI: 10.12116/j.issn.1004-5619.2019.05.010

• 论著 • 上一篇    下一篇

大鼠过劳模型心脏Cx43和Cx45的表达

杨博帆1， 史婧卓1， 李晶1， 潘玉蓬1， 肖宁1， 余彦耿2， 张付2， 王慧君1， 李冬日1   

  1. 1. 南方医科大学法医学院，广东 广州 510515； 2. 广东省公安厅 公安部法医病理学重点实验室，广东 广州 510050
  • 发布日期:2019-10-25 出版日期:2019-10-28
  • 通讯作者: 李冬日，男，博士，副教授，主要从事法医病理学、法医临床学的教学、科研和鉴定；E-mail：m13826034910@163.com
  • 作者简介:杨博帆（1995—），男，主要从事法医学教学及科研；E-mail：1162354297@qq.com
  • 基金资助:
    大学生创新创业训练计划资助项目（201612121085，201812121244）；公安部重点实验室开放课题资助项目（GAFYBL201601）

Expression of Cx43 and Cx45 in Cardiomyocytes of an Overworked Rat Model

YANG Bo-fan1, SHI Jing-zhuo1, LI Jing1, PAN Yu-peng1, XIAO Ning1, YU Yan-geng2, ZHANG Fu2, WANG Hui-jun1, LI Dong-ri1   

  1. 1. School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China; 2. Key Laboratory of Forensic Pathology, Ministry of Public Security, Guangdong Provincial Public Security Department, Guangzhou 510050, China
  • Online:2019-10-25 Published:2019-10-28

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摘要： 目的 研究过劳应激对心肌细胞连接蛋白（connexin，Cx） 43和Cx45的表达及其对心脏功能的影响。 方法 将实验动物分为对照组、过劳1月组和过劳2月组，过劳组通过强制游泳建立过劳模型。运用Western印迹法对实验动物心肌组织内Cx43和Cx45的表达情况进行检测，同时对相应心肌组织进行苏木精-伊红（hematoxylin-eosin，HE）和Masson染色并进行组织学观察。 结果 Western印迹法结果显示，与对照组相比，过劳大鼠心肌组织内Cx43表达降低、Cx45表达升高。HE染色和Masson染色结果显示，过劳大鼠心肌纤维出现肥大、断裂并伴有间质纤维组织增生。 结论 过劳应激反应可能作为一种独立因素对心脏功能产生影响，甚至引起心力衰竭或心律失常导致死亡。

关键词: 法医病理学；心脏损伤；连接蛋白43；连接蛋白45；模型, 动物；过劳死；大鼠

Abstract: Objective To study the effect of overwork stress response on the expression of connexin 43（Cx43） and connexin 45（Cx45） in cardiomyocytes and on cardiac function. Methods The experimental animals were divided into control group, overworked 1-month group and overworked 2-month group. A overworked rat model was established by forcing swimming of overworked group. The expressions of Cx43 and Cx45 in myocardial tissues of experimental animals were detected by Western blotting, while the corresponding myocardial tissues were stained with hematoxylin-eosin （HE） staining and Masson’s staining, then histologically observed. Results Western blotting results showed that, compared with the control group, Cx43 expression in myocardial tissues of overworked rats decreased while Cx45 expression increased. HE staining and Masson’s staining results showed that hypertrophy, rupture and interstitial fiber tissue hyperplasia were observed in myocardial fibers of overworked rats. Conclusion Overwork stress response may affect cardiac function as an independent factor and may even cause heart failure or arrhythmias and lead to death.

Key words: forensic pathology, heart injuries, connexin 43, connexin 45, models, animal, karoshi, rats