法医学杂志

法医学杂志 ›› 2023, Vol. 39 ›› Issue (2): 151-160.DOI: 10.12116/j.issn.1004-5619.2022.321101

• 论著 • 上一篇    下一篇

基于微分段技术分析单根头发中42种精神活性物质

纪佼佼1,2(), 徐多麒1, 向平1, 严慧1(), 沈敏1()   

  1. 1.司法鉴定科学研究院 上海市法医学重点实验室 司法部司法鉴定重点实验室 上海市司法鉴定专业技术服务平台，上海 200063
    2.复旦大学基础医学院，上海 200032
  • 收稿日期:2022-11-07 发布日期:2023-06-06 出版日期:2023-04-25
  • 通讯作者: 严慧,沈敏
  • 作者简介:严慧，女，博士，副研究员，主要从事法医毒物分析研究；E-mail：yanh501@163.com
    沈敏，女，研究员，博士研究生导师，主要从事法医毒物分析研究；E-mail：shenm@ssfjd.cn
    纪佼佼（1995—），女，博士研究生，主要从事法医毒物分析研究；E-mail：845999211@qq.com
  • 基金资助:
    国家自然科学基金资助项目(81772022);司法部司法鉴定重点实验室资助项目;上海市法医学重点实验室资助项目(21DZ2270800);上海市司法鉴定专业技术服务平台资助项目

Analysis of Forty-Two Psychoactive Substances in a Single Hair by Micro-Segmental Technique

Jiao-jiao JI1,2(), Duo-qi XU1, Ping XIANG1, Hui YAN1(), Min SHEN1()   

  1. 1.Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China
    2.School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
  • Received:2022-11-07 Online:2023-06-06 Published:2023-04-25
  • Contact: Hui YAN,Min SHEN

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摘要：

目的 建立一种基于单根头发微分段技术的LC-MS/MS分析方法，并对42种精神活性物质在0.4 mm头发节段中的检测进行验证。 方法 将每根头发剪成0.4 mm长的片段，超声提取，并在含二硫苏糖醇的提取液中浸泡。流动相A为含20 mmol/L乙酸铵、0.1%甲酸、5%乙腈的水溶液，流动相B为乙腈，采用电喷雾离子源正离子模式，在多反应监测模式下采集数据。 结果 单根头发中42种精神活性物质在各自线性范围内线性关系良好（r>0.99），检出限为0.2~10 pg/mm，最低定量限为0.5~20 pg/mm，日内、日间精密度为1.5%~12.7%，日内、日间准确度为86.5%~109.2%，提取回收率为68.1%~98.2%，基质效应为71.3%~111.7%。应用该方法对单次服用唑吡坦28 d的志愿者头发样本进行分析，5根头发中唑吡坦的检出位置位于近根端1.08~1.60 cm，浓度范围为0.62~20.5 pg/mm。 结论 单根毛发微分段分析技术可应用于药物辅助性犯罪案件的调查。

关键词: 法医学, 毒物分析, 精神活性物质, 微分段, 单根头发, 液相色谱-串联质谱, 唑吡坦, 药物辅助性犯罪

Abstract:

Objective To establish an LC-MS/MS method based on single hair micro-segmental technique, and verify the detection of 42 psychoactive substances in 0.4 mm hair segments. Methods Each piece of single hair was cut into 0.4 mm segments and extracted by sonication and the segments were immersed in dithiothreitol-containing extraction medium. Mobile phase A was the aqueous solution containing 20 mmol/L ammonium acetate, 0.1% formic acid, and 5% acetonitrile. Mobile phase B was acetonitrile. An electrospray ionization source in positive ion mode was used for data acquisition in multiple reaction monitoring （MRM） mode. Results The 42 psychoactive substances in hair had a good linear relationship within their respective linear ranges （r>0.99）, the limits of detection were 0.2-10 pg/mm, the limits of quantification were 0.5-20 pg/mm, the intra-day and inter-day precisions were 1.5%-12.7%, the intra-day and inter-day accuracies were 86.5%-109.2%, the recovery rates were 68.1%-98.2%, and the matrix effects were 71.3%-111.7%. The method was applied to hair samples collected from one volunteer at 28 d after a single dose of zolpidem, with zolpidem detected in 5 hairs was 1.08-1.60 cm near the root tip, and the concentration range was 0.62-20.5 pg/mm. Conclusion The micro-segmental technique of single hair analysis can be applied to the investigation of drug-facilitated sexual assault cases.

Key words: forensic medicine, toxicological analysis, psychoactive substance, micro-segmentation, single hair, liquid chromatography-tandem mass spectrometry （LC-MS/MS）, zolpidem, drug-facilitated sexual assault

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