用荧光抗体法作血痕的种属鉴定

法医学杂志 ›› 1990, Vol. 0 ›› Issue (1): 33-34.

用荧光抗体法作血痕的种属鉴定

蔡锐波;郭景元;

中山医科大学法医系,中山医科大学法医系,

发布日期:1990-02-25 出版日期:1990-02-28

The Species Identification of Bloodstain by the Immunofluorescence Test

DEPARTMENT OF FORENSIC SEROLOGY SUN YAT-SEN UNIVERSITY OF MEDICAL SCICESEN CHAI RUIBO GUO JINGYUAN

Online:1990-02-25 Published:1990-02-28

摘要/Abstract

摘要： <正> 血痕的种属鉴定,最早是应用抗人血清作环状沉淀试验,后来使用特异性更好的抗人血红蛋白沉淀素血清替代抗人血清作各种沉淀试验.此外,还有应用抗人球蛋白消耗试验、间接血凝试验法、胶乳载体试验法.近来,随着免疫学实验技术的发展,应用酶联免疫技术等检测人血液中各种特异组分作血痕的种属鉴识.本文使用荧光标记抗体检测人血痕中的IgG组分,作人血和动物血的鉴别,取得较好结果,现将实验报告如下:

Abstract: Using sheep antihuman IgG serum labelled with fluorescein-iso-thiocyanate ,the human IgG in bloodstains could be found. The positive result could be acquired even with a few micro-fibrin torn from a 1-cm long sauge fiber stained with 2-ul of 1:100 diluted blood (1:200 diluted serum). That means the stains containing only one-thousandth micro gram of serum protain could be identified by this method. 127 human bloodstains within five years old and eleven bloodstains of eleven kinds of animals were tested and all the results were correct.The blind test of 90 bloodstains (human, 55, animai,35) got 88 correct conclusion (97.78%) and 2 misjudgement(2.22%) . The species identification of blood stain using the immunofluorescence techque is a nice me-theod with high sensitivety and specifificity.

蔡锐波;郭景元;. 用荧光抗体法作血痕的种属鉴定[J]. 法医学杂志, 1990, 0(1): 33-34.

DEPARTMENT OF FORENSIC SEROLOGY SUN YAT-SEN UNIVERSITY OF MEDICAL SCICESEN CHAI RUIBO GUO JINGYUAN. The Species Identification of Bloodstain by the Immunofluorescence Test[J]. , 1990, 0(1): 33-34.