DNA指纹的个体特异性和细胞稳定性研究

法医学杂志 ›› 1990, Vol. 0 ›› Issue (2): 8-11.

DNA指纹的个体特异性和细胞稳定性研究

蓝翎;张小为;段秉章;霍振义;

北京市刑事科学技术研究所,北京市刑事科学技术研究所,北京市刑事科学技术研究所,北京市刑事科学技术研究所,

发布日期:1990-04-25 出版日期:1990-04-28

The study of individual specificity and somatic stability of DNA fingerprints

LAN LIN; ZHANG XIAO-WEI; DUAN BING-ZHANG; HOU ZHENG-YI BEIJING FORENSIC SCIENCE INSTITUTE

Online:1990-04-25 Published:1990-04-28

摘要/Abstract

摘要： <正> 人类基因组的许多高度可变区域能够与小卫星探针杂交,而同时被检测出来,小卫星探针由串联重复的"核心"顺序构成.采用Southern印迹杂交获得的DNA指纹图包含多条高度可变的DNA片段杂交带,这些带主体细胞和生殖细胞中都表现出稳定性,而且安全具有个体特异性.本文用小卫星探针33.15,调查了北京地区的60名无关个体,

Abstract: A blind trial involving 60 blood samples has been successfully carried out. By using minisatellite probe 33.15 we have obtained satisfactory DNA fingerprints. After statistical treatment the probability of individual specificity is calculated as 5.6×10~(-12). Futhermore, we have studied blood, semen and bloodstain of the same individual by probe 33.6, resulting identica DNA fingerprints. It shows somatic and germ-line stability and completely specific to individual. The viewpoint of international standardization of DNA fingerprints has been first proposed in this paper.

Key words: DNA fingerprints, minisatellite probe, hypervariable region, probability of individual specificity

蓝翎;张小为;段秉章;霍振义;. DNA指纹的个体特异性和细胞稳定性研究[J]. 法医学杂志, 1990, 0(2): 8-11.

LAN LIN;ZHANG XIAO-WEI;DUAN BING-ZHANG;HOU ZHENG-YI BEIJING FORENSIC SCIENCE INSTITUTE. The study of individual specificity and somatic stability of DNA fingerprints[J]. , 1990, 0(2): 8-11.