法医学杂志

法医学杂志 ›› 1998, Vol. 0 ›› Issue (1): 18-19+6.

• 论文 • 上一篇    下一篇

人体组织两种简易保存法DNA抽提效果的比较研究

徐宏斌   

  1. 皖南医学院生化教研室!安徽芜湖,241001
  • 发布日期:1998-02-25 出版日期:1998-02-28

Comparative studiy on DNA abstraction of human tissues preseved by two different methods

XU HONGBIN (DEPARTMENT OF BIOCHEMISLRY WANNANMEDICAL COLLEGE. WUHU 241001)   

  • Online:1998-02-25 Published:1998-02-28

摘要： 比较研究人体不同组织在不同保存条件下DNA抽提效果。取冷冻保存和50％乙醇固定保存的各种组织，用有机溶剂抽提法提取DNA。用50％乙醇固定保存的人体不同组织在校长时期内仍可获得与新鲜冷冻组织相近的DNA抽提效果，经卡方检验二者无明显差异（X2≤0．21，P≥0．995）。乙醇保存法简便易行，DNA产量高，效果好，适用于各种特殊情况下的组织保存。

关键词: 人体组织, 50％乙醇固定, DNA抽提

Abstract: Comparative studies have been made on the effect ofDNA abstraction of different human tissues preserved bydifferent methods. The results showed that differenthuman tissues preserved in 50% alcohol may havesimilar DNA abstraction effect for a relatively long timeas frozen tissues. They have no significant differenceThis preservation method has theadvantage of simplicity, high DNA output and goodeffect, and is suitable for preserving tissues underspecial circumstances.

Key words: human tissue, alcohol preservation, DNA abstraction