法医学杂志

法医学杂志 ›› 1998, Vol. 0 ›› Issue (1): 8-9+61.

• 论文 • 上一篇    下一篇

VNTR D17530位点多态性在亲子鉴定中的应用

冯明亮,冯哲,陆琼,张雁征,杨颖,嵇月华,陈仁彪   

  1. 上海市输血研究所免疫遗传研究室!上海,200051,上海市输血研究所免疫遗传研究室!上海,200051,上海市输血研究所免疫遗传研究室!上海,200051,上海市输血研究所免疫遗传研究室!上海,200051,上海市输血研究所免疫遗传研究室!上海,200051,上海市输血研究所免疫遗传研究室!上海,2000
  • 发布日期:1998-02-25 出版日期:1998-02-28

Application of VNTR D17S30 locus polymorphism in the paternity test

FENG MINGLIANG, FENG ZHE LU QING. ZHANGZ-HANG,YANG YING, JI YUEHUA, CHEN RENBIAO (IMMUNO-GENTETICSLABORATORY, SHANGHAI INSTITUTUTE OF BLOOD TRANSFUSION,SHANGHAI BLOOD CENTER, SHANGHAI 200051)   

  • Online:1998-02-25 Published:1998-02-28

被引次数

6

摘要： 运用微量热启动PCR技术，对20例正常家系的遗传学分析，证实D17530位点的遗传符合孟德尔遗传规律，表现为简单的共显性遗传。同时，对100例亲子鉴定案例进行回顾性分析研究，证明D17S30位．在多态性可应用于我国法医学亲子鉴定。根据D17S30位点基因频率估算的排除概率（74．04％）与其实际排除能力（80．00％）无显著差异。在15倒排除亲子关系的案例中，有2例由D17S30位点单独取得排除证据。D17S30位点是法医学上有重要意义的遗传学标记，可用于亲子鉴定。

关键词: VNTRD17S30, 亲子鉴定, 排除概率

Abstract: A sensitive and rapid PCR-based technique was adoptedto genotype the VNTR D17S30 locus. It was confirmedthrough the genetic analysis of 20 normal families thatthe inheritance of D17S30 locus coincides withMendelian law as simple co-dominant. Retrospective analysis of 100 Paternity cases demonstrated thatD17S30 locus could be used in forensic paternity test inour counts. The exclusion probability estimated fromallele frequencies of D17S30 locus (74.04%) does notdiffer significantly from the observed rate of exclusion(80.00%) in these cases. In all excluded paternity casesthere are two in which the exclusion evidence is solelyprovided by the D17530 locus.

Key words: VNTR D17S30, paternity test, probability of exclusion