法医学杂志 ›› 1999, Vol. 0 ›› Issue (4): 201-203+.

• 论文 • 上一篇    下一篇

HLA-DRB1位点的PCR-SSP分型在亲子鉴定中的应用研究

刘利民,梁健,王保捷,丁梅,李建平,李春梅,肖仰哲,贾静涛   

  1. 中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学第一临床学院器官移植科!辽宁沈阳,110001,中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学法医
  • 发布日期:1999-08-25 出版日期:1999-08-28

HLA-DRB1 Typing by PCR-sequence specific primers for paternity determination

LIU LIMIN; LIANG JIAN; WANG BAOJIC, ET AL. (DEPARTMENTOF FORENSIC MEDICINE, CHINA MEDICAL UNIVERSITY,SHENYANG 110001, P. R. CHINA)   

  • Online:1999-08-25 Published:1999-08-28

摘要: 应用PCR-SSP(PCRamplificationwithsequencespecificprimer)方法将HLAⅡ类DRB1位点基因分型应用于亲权鉴定。对42例亲子鉴定案例进行分析研究的结果表明,本方法简单、快速、结果可靠,且具有较高的非父排除概率(66.3%),不仅适用于法医学亲手鉴定和个人识别,亦可应用于移植配型、HLA相关疾病及人类遗传学研究。

关键词: 亲子鉴定, PCR-SSP, HLA-DRB1基因

Abstract: HLA-DRB1 typing by PCR - SSP technique was first used for patemity testing. The analysis of 42 paternity cases shows that this identification method is simple, Thst and reliable and has high exclusion probability of paternity (66. 3% ). This method can also be applied to the study of transplantation, HLA associated disease and anthropogenesis

Key words: paternity testing, PCR - SSP, HLA - DRB1 gene