法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (1): 1-3+99.

• 论文 •    下一篇

大鼠脑液压冲击伤caspase-8的表达

杨静;王晔;陈晓刚;彭其毅;李雷波;刘敏;   

  1. 四川大学华西基础医学与法医学院法医病理教研室,四川大学华西基础医学与法医学院法医病理教研室,四川大学华西基础医学与法医学院法医病理教研室,四川大学华西基础医学与法医学院法医病理教研室,济南市公安局刑警大队,四川大学华西基础医学与法医学院法医病理教研室 四川成都610041,四川成都610041,四川成都610041,四川成都610041,山东济南250052,四川成都610041
  • 发布日期:2006-02-25 出版日期:2006-02-28

Expression of Caspase-8 after Brain Injury from Fluid Percussion in Rats

YANG JING1, WANG YE1, CHEN XIAO-GANG1, PENG QI-YI1, LI LEI-BO2, LIU MIN1 (1.WEST CHINA COLLEGE OF PRECLINICAL MEDICINE AND FORENSIC SCIENCES OF SICHUAN UNIVERSITY,CHENDU 610041,CHINA; 2. JINAN PUBLIC SECURITY BUREAU,JINAN 250052,CHINA)   

  • Online:2006-02-25 Published:2006-02-28

摘要： 目的探讨大鼠脑损伤后caspase-8表达情况,为脑损伤的诊断及损伤时间推断提供依据。方法建立大鼠脑液压冲击伤模型。用免疫组化与图像分析技术分别检测伤后15,30min和1,3,6,12h及1,4,7,14d大鼠皮质、丘脑、海马等部位caspase-8的表达。结果发现伤后30min大脑皮质和海马caspase-8开始出现表达,随时间增加其表达亦逐渐增加,伤后3h显著增加,24h达到高峰,4d后逐渐减少,14d基本恢复正常;而丘脑在伤后1h才开始出现阳性表达,伤后6h出现明显表达,24h达到高峰,4d后逐渐减少,14d基本恢复正常。同时发现损伤对侧海马及丘脑出现相应的变化规律。结论caspase-8阳性表达的变化规律可作为脑损伤诊断及损伤时间推测的指标之一。

关键词: 液压冲击伤, 免疫组化, caspase-8

Abstract: Objective To provide the evidence of the relationship between brain injury and the time of injury. Methods Rats were contused on brain by fluid percussion, then were killed after injury for 15min, 30 min, 1,3,6,12 h, and 1,4,7,14 d respectively. The expression of caspase-8 were detected by immunohistochemical technology on rat brain section and the results were assessed by image analysis system in the cerebral cortex, thalamus, and hippocampus. Results The expression of caspase-8 in cortex and hippocampus could be detected in 30min after injury, increased significantly in 3h, reached apex in 1d after injury, remained 4d before decreased. In addition, the expression of caspase-8 can be detected in 1h after injury and reached apex in 1d after injury, and remained 4d then reduced. Conclusion It seems that the expression of caspase-8 should be a useful target for diagnosis of early brain injury.

Key words: fluid percussion brain injury, immunohistochemical technology, caspase-8