法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (1): 52-54+5.

• 论文 • 上一篇    下一篇

生物检材中吗啡类生物碱的LC-MS/MS分析

向平;沈敏;沈保华;马栋;卜俊;姜宴;卓先义;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,复旦大学上海医学院,司法部司法鉴定科学技术研究所 上海200063,上海200063,上海200063,上海200063,上海200063,上海200063
  • 发布日期:2006-02-25 出版日期:2006-02-28

Determination of Opiates in Biological Human Samples by Liquid Chromatography-Tandem Mass Spectrometry

XIANG PING1, SHEN MIN1, SHEN BAO-HUA1, MA DONG1, BU JUN1, JIANG YAN2, ZHUO XIAN-YI1 (1.INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE, P.R.CHINA; 2. SHANGHAI MEDICAL COLLEGE, FUDAN UNIVERSITY, SHANGHAI 200032, CHINA)   

  • Online:2006-02-25 Published:2006-02-28

被引次数

74

摘要： 目的针对滥用药物分析鉴定实践中亟待解决的问题,开展LC-MS/MS分析生物检材中吗啡类生物碱的应用研究。方法满足不同的鉴定需要,分别建立血液、尿液、唾液和头发等生物检材的样品前处理方法,确定同时分析海洛因、单乙酰吗啡、吗啡、可待因、乙酰可待因、二氢可待因酮和氢吗啡酮等吗啡类生物碱的LC-MS/MS方法。将方法应用于实际案例。结果所建立的方法对吗啡类生物碱分离良好。尿液稀释法、尿液提取法和头发中吗啡的最低检测限(LOD)分别为10ng/mL、0.01ng/mL和0.01ng/mg。结论所建立的方法简便、快速、特异性强、灵敏度高。目标物中加入二氢可待因酮和氢吗啡酮扩大了方法的实用范围。

关键词: LC-MS/MS, 吗啡类生物碱, 稀释, 唾液, 灵敏度

Abstract: Objective Liquid chromatography-tandem mass spectrometry(LC-MS/MS) was developed for the determination of opiates in biological samples according to the emerging problem in drugs abuse. Methods Opiates such as heroin, 6-acetylmorphine, morphine, codeine, acetylcodeine, hydrocodone and hydromorphone were isolated from human blood, urine, oral fluid and hair using a simple extraction and consequently analyzed using LC-MS/MS. The method was evaluated by real cases. Results The mobile phase give the optimum separation for opiates. The detection limit of morphine in urine with dilution and liquid-liquid extraction and in hair is 10ng/mL, 0.01 ng/mL and 0.01 ng/mg, respectively. Conclusion The method is simple and rapid, offering superior sensitivity and selectivity for opiates. The target compounds comprising hydrocodone and hydromorphone enlarge the applied area.

Key words: LC-MS/MS, opiates, dilution, oral fluid, sensitivity