法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (2): 88-89+9.

• 论文 • 上一篇    下一篇

大鼠脑震荡后GFAP的表达

张纯兵;李永宏;   

  1. 皖南医学院法医系,皖南医学院法医系 安徽芜湖241001,安徽芜湖241001
  • 发布日期:2006-04-25 出版日期:2006-04-28

The Expression of GFAP after Brain Concussion in Rats

ZHANG CHUN-BING, LI YONG-HONG (DEPARTMENT OF FORENSIC MEDICINE, WANNAN MEDICAL COLLEGE, WUHU 241001,CHINA )   

  • Online:2006-04-25 Published:2006-04-28

被引次数

6

摘要： 目的探讨脑震荡后GFAP在脑内的时间性变化规律,为脑震荡后经过时间的法医学鉴定提供理论依据。方法54只SD大鼠按不同损伤时间分成3,6,12,24h和2,4,7,10d及对照组,用单摆式机械打击装置建立大鼠脑震荡模型,用免疫组织化学技术观察大鼠脑震荡后GFAP在伤后不同时间的表达情况。结果正常大鼠可见少量GFAP阳性细胞,脑震荡后6h后,随伤后经过时间的延长,GFAP表达逐渐增强,阳性细胞数目增多,胞体增大,突起增粗、增多,染色加深,至伤后7d达高峰。结论GFAP的检测可作为法医病理学诊断脑震荡的依据和推断脑震荡后经过时间的参考指标。

关键词: 脑震荡, 胶质纤维酸性蛋白(GFAP), 免疫组织化学, 损伤时间

Abstract: objective To study the expression of GFAP and pathologic changes after rats brain concussion, so that to provide evidence on brain concussion for forensic identification. Methods Forty-five SD rats were divided into 3,6,12,24 h and 2,4,7,10 d and normal control groups in terms of different wounding time after brain concussion model established, and the expression of GFAP after rats brain concussion were then observed by using SP immunohistochemical method. Results In normal control brain, low-level GFAP expressions could be observed. After six hours′ brain concussion, GFAP positive cells increased obviously. The trend reached to the peak at 7d, partly declined at 10 d, then decreased gradually. Conclusion Brain concussion induced the expression of GFAP. The detection of GFAP could be useful for diagnosis of brain concussion on forensic pathology, and could be a reference index for timing of injury after brain concussion

Key words: brain concussion, glial fibrillary acidic protein (GFAP), immunohistochemistry, injury time