法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (6): 411-413+.

• 论文 • 上一篇    下一篇

免疫荧光及双抗体夹心ELISA在人狂犬病检测中的应用

孙来晶;徐葛林;王华林;吴杰;胡志红;周亦武;   

  1. 华中科技大学同济医学院法医学系,武汉生物制品研究所基因工程室,中国科学院武汉病毒研究所病毒学国家重点实验室,武汉生物制品研究所基因工程室,中国科学院武汉病毒研究所病毒学国家重点实验室,华中科技大学同济医学院法医学系 湖北武汉430030 中国科学院武汉病毒研究所病毒学国家重点实验室,湖北武汉430071,湖北武汉430060,湖北武汉430071,湖北武汉430060,湖北武汉430071,湖北武汉430030
  • 发布日期:2007-12-25 出版日期:2007-12-28

Application of Immunofluorescence and Sandwich ELISA with Double-antibodies in Detection of Human Rabies

SUN LAI-JING1,2, XU GE-LIN3, WANG HUA-LIN2, WU JIE3, HU ZHI-HONG2, ZHOU YI-WU1 (1.DEPARTMENT OF FORENSIC MEDICINE, TONGJI MEDICAL COLLEGE, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY, WUHAN 430030, CHINA; 2.STATE KEY LABORATORY OF VIROLOGY, WUHAN INSTIT   

  • Online:2007-12-25 Published:2007-12-28

被引次数

8

摘要： 目的探讨免疫荧光和双抗体夹心ELISA在人狂犬病检测中的应用。方法4例临床诊断狂犬病死亡患者的大脑、小脑、脑干、海马,分别于冰箱和甲醛固定后保存,用上述方法检测。感染狂犬病毒CVS株的鼠脑组织和急性胰腺炎死者脑组织分别作为阳性和阴性对照。结果-70℃保存的人脑和阳性对照狂犬病毒均为阳性,甲醛固定的人脑和阴性对照均为阴性。结论免疫荧光和双抗体夹心ELISA可用于人狂犬病检测,但脑组织要低温保存,不能用甲醛固定。

关键词: 人狂犬病, 法医学鉴定, 免疫荧光, 双抗体夹心ELISA

Abstract: Objective To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies. Methods The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at-70 ℃ or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute pancreatitis was used as a negative control. Results Rabies virus was detected in the tissues kept in freezer at-70 ℃ and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control. Conclusion Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution.

Key words: human rabies, forensic identification, immunofluorescence, Sandwich ELISA with double-antibodies