法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (6): 438-440.

• 论文 • 上一篇    下一篇

人骨骼肌线粒体DNA 4977片段缺失与年龄的相关性调查

张幼芳;   

  1. 浙江警察学院 浙江杭州310053
  • 发布日期:2007-12-25 出版日期:2007-12-28

Age-dependent Mitochondrial DNA 4977bp Depletion in Human Skeletal Muscle

ZHANG YOU-FANG (ZHEJIANG POLICE COLLEGE, HANGZHOU 310053, CHINA)   

  • Online:2007-12-25 Published:2007-12-28

被引次数

4

摘要： 目的探讨人骨骼肌组织中线粒体DNA 4977bp片段的缺失情况及其与年龄的相关性。方法收集105例不同年龄死者的骨骼肌组织,抽提mtDNA,通过PCR、琼脂糖紫外凝胶成像等技术,测定不同年龄组线粒体DNA 4977bp片段的缺失情况。结果在105例样品中,不同年龄组(0～9、10～19、20～29、30～39、40～49、50～59、60～69、70～79、80～89、90～99岁)mtDNA4977片段缺失的频率分别为:0、0、0.003%、0.011%、0.015%、0.033%、0.038%、0.062%、0.069%、0.091%。结论人骨骼肌线粒体DNA 4977bp片段的缺失频率随着年龄的增长而增高,各年龄组之间具有显著差异(P<0.01)。该缺失片段的检测对于软组织年龄推测具有一定指导意义。

关键词: 线粒体DNA, 缺失, 年龄推断

Abstract: Objective To explore depletion of human mitochondrial DNA 4977-bp and its relation with aging. Methods Total DNA (nuclear and mtDNA) was extracted from 100 mg muscle tissue. UV light illumination of ethidium bromide-stained PCR products was used to study the depletion of mtDNA (wild-type or mutant). Results The proportions of mtDNA depletion in human skeletal muscle could be determined. The frequency of mtDNA 4977-bp depletion in different age groups (0-9, 10-19, 20-29, 30-39, 40-49, 50-59, 60-69, 70-79, 80-89, 90-99) was: 0%, 0%, 0.003%, 0.011%, 0.015%, 0.033%, 0.038%, 0.062%, 0.069%, and 0.091%, respectively. Conclusion Our findings suggest that the frequency of the mtDNA4977 depletion in human skeletal muscle increases with age. It might be useful for human age estimation.

Key words: mitochondrial DNA, deletion, age estimation