法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (1): 1-5.

• 论文 •    下一篇

大鼠心肌傅里叶变换红外光谱变化与死亡时间的关系

黄平;苏昌沛;李石松;邢博;托娅;鲁庆阳;柯咏;王振原;   

  1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室;西安交通大学医学院法医学系;永春县公安局;安康市公安局汉滨区分局;
  • 发布日期:2010-02-25 出版日期:2010-02-28

Estimation of Postmortem Interval Using FTIR Spectroscopy in Rats' Cardiac Muscle

HUANG PING1,2,SU CHANG-PEI3,LI SHI-SONG4,XING BO2,TUO YA2,LU QING-YANG2,KE YONG2,WANG ZHEN-YUAN2(1.SHANGHAI KEY LABORATORY OF FORENSIC SCIENCE,INSTITUTE OF FORENSIC SCIENCE,MINISTRY OF JUSTICE,P.R.CHINA,SHANGHAI 200063,CHINA;2.DEPARTMENT OF FORENSIC SCIEN   

  • Online:2010-02-25 Published:2010-02-28

被引次数

36

摘要： 目的应用傅里叶变换红外(Fourier transform infrared,FTIR)光谱技术分析大鼠死后心肌组织随死亡时间推移的化学降解过程,为死亡时间推断的研究提供新的途径与方法。方法大鼠断颈处死后置于(20±2)℃环境,在不同死亡时间点提取大鼠左心室心肌组织,并使用FTIR光谱仪测定不同化学基团的变化。结果随着死亡时间的推移,大鼠心肌组织FTIR光谱的主要吸收峰位没有明显变化,而其吸光度随着死亡时间增加呈现出增加、下降和稳定3种不同的变化方式,且不同峰的吸光度比显示了相似的时间变化趋势。结论心肌组织可以作为FTIR光谱技术分析死亡时间的适用检材。

关键词: 法医病理学, 心肌, 谱学, 傅里叶变换红外, 死亡时间推断, 大鼠

Abstract: Objective FTIR(Fourier transform infrared) spectroscopy was applied to observe the process of postmortem degradation in rats’cardiac muscle and provided a new method for the estimation of post-mortem interval(PMI).Methods The rats were sacrificed by cervical dislocation and the bodies were kept in a controlled environmental chamber set at(20±2) ℃.The FTIR spectra was applied to measure the changes of different chemical group from rats’ left ventricle muscle at the different time point postmortem.Results There were not obvious changes for the main FTIR absorbance peaks.But the different FTIR absorbance at the wave-number(cm-1) indicated the three types: increase,decrease,stable.The various absorbance ratios al-so demonstrated the similar changes.Conclusion FTIR spectroscopy may be potentially used as an effec-tive method for estimating PMI in forensic practice using cardiac muscle tissue.

Key words: forensic pathology, myocardium, spectroscopy, Fourier transform infrared, estimation of post-mortem interval, rats