法医学杂志

法医学杂志 ›› 2022, Vol. 38 ›› Issue (1): 114-118.DOI: 10.12116/j.issn.1004-5619.2021.410605

所属专题： 水中尸体研究专题

• 技术与应用 • 上一篇    下一篇

MD-VF-Auto SEM法与浮游生物基因复合扩增体系在溺死诊断中的应用比较

张健苗1(), 林添春1, 廖振瑜1, 杜宇坤2, 余仲昊2, 刘景建3, 伍赛群4, 康晓东5, 徐曲毅5, 石河5, 赵建5, 刘超5(), 谷党恩1()   

  1. 1.广州市公安局花都区分局，广东 广州 510800
    2.南方医科大学法医学院，广东 广州 510515
    3.昆明医科大学法医学院，云南 昆明 650500
    4.广东省公安厅，广东 广州 510030
    5.广州市刑事科学技术研究所 法医病理学公安部重点实验室，广东 广州 510442
  • 收稿日期:2021-06-14 发布日期:2022-02-25 出版日期:2022-02-28
  • 通讯作者: 刘超,谷党恩
  • 作者简介:谷党恩，男，副主任法医师，主要从事法医病理学检验和溺死诊断研究；E-mail：gudn@sohu.com
    刘超，男，博士，主任法医师，主要从事个体识别及死因鉴定研究；E-mail：liuchaogzf@163.com
    张健苗（1987—），男，主检法医师，主要从事法医学检验鉴定及研究；E-mail：296770581@qq.com
  • 基金资助:
    广州市科技计划资助项目(2019030001);法医病理学公安部重点实验室开放课题资助项目(GAFYBL201909);公安部科技强警基础工作专项资助项目(2020GABJC38)

Comparison of Application of MD-VF-Auto SEM Method and Plankton Gene Multiplex PCR System in the Diagnosis of Drowning

Jian-miao ZHANG1(), Tian-chun LIN1, Zhen-yu LIAO1, Yu-kun DU2, Zhong-hao YU2, Jing-jian LIU3, Sai-qun WU4, Xiao-dong KANG5, Qu-yi XU5, He SHI5, Jian ZHAO5, Chao LIU5(), Dang-en GU1()   

  1. 1.Huadu District Branch of Guangzhou Public Security Bureau, Guangzhou 510800, China
    2.School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China
    3.College of Forensic Medicine, Kunming Medical University, Kunming 650500, China
    4.Guangdong Provincial Public Security Department, Guangzhou 510030, China
    5.Guangzhou Forensic Science Institute & Key Laboratory of Forensic Pathology, Ministry of Public Security, Guangzhou 510442, China
  • Received:2021-06-14 Online:2022-02-25 Published:2022-02-28
  • Contact: Chao LIU,Dang-en GU

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摘要： 目的

比较微波消解-真空抽滤-自动扫描电子显微镜（microwave digestion - vacuum filtration - automated scanning electron microscopy，MD-VF-Auto SEM）法与浮游生物基因复合扩增体系在溺死诊断中的应用效果。

方法

采用MD-VF-Auto SEM法和浮游生物基因复合扩增体系分别对10例非溺死案例和50例溺死案例的肺、肝、肾组织进行检测分析，比较两种方法在各组织中的阳性检出率。

结果

溺死案例使用MD-VF-Auto SEM法检验的硅藻阳性率为100%，在6例非溺死案例的肝、肾组织中检出少量硅藻。浮游生物基因复合扩增体系的阳性率为84%，非溺死案例的肺、肝、肾组织结果均为阴性。两种方法检测结果相比，肝、肾组织的阳性率和总阳性率差异均具有统计学意义（P<0.05），而肺组织阳性率差异无统计学意义（P>0.05）。

结论

MD-VF-Auto SEM法对硅藻检验具有较高的灵敏度，而浮游生物基因复合扩增体系还可检出硅藻外的其他浮游生物。两种方法相结合，可为溺死诊断提供更可靠的依据。

关键词: 法医病理学, 溺死, 硅藻检验, 浮游生物, 复合扩增体系, 微波消解-真空抽滤-自动扫描电子显微镜法

Abstract: Objective

To compare the application effect of microwave digestion - vacuum filtration - automated scanning electron microscopy （MD-VF-Auto SEM） method and plankton gene multiplex PCR system in the diagnosis of drowning.

Methods

Lung, liver and kidney tissue of 10 non-drowning cases and 50 drowning cases were prepared for further MD-VF-Auto SEM method analysis and plankton gene multiplex PCR system analysis. The positive detection rate of the two methods in each tissue was calculated.

Results

The positive rate of the MD-VF-Auto SEM method detecting diatoms in drowning cases was 100%, and few diatoms were detected in the liver and kidney tissues of 6 non-drowning cases. By using the plankton gene multiplex PCR system, the diatom positive rate of drowning cases was 84%, and all the non-drowning cases were negative. There were significant differences in the positive rate of the liver, kidney tissues between MD-VF-Auto SEM method and plankton gene multiplex PCR system （P<0.05）, as well as the total positive rate of cases. However, no significant differences were found in the positive rates of lung tissues （P>0.05）.

Conclusion

MD-VF-Auto SEM method is more sensitive than plankton gene multiplex PCR system in diatom test. But the plankton gene multiplex PCR system can also detect plankton other than diatoms. Combination of the two methods can provide a more reliable basis for the diagnosis of drowning.

Key words: forensic pathology, drowning, diatom test, plankton, multiplex PCR system, microwave digestion-vacuum filtration-automated scanning electron microscopy method

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