法医学杂志

法医学杂志 ›› 1995, Vol. 0 ›› Issue (2): 0-0.

• 论文 •    下一篇

高效液相色谱法相对保留时间和峰高比在毒物筛选中的应用

张玉荣，刘琦，孙志清   

  1. 上海市刑事科学技术研究所
  • 发布日期:1995-04-25 出版日期:1995-04-28

Application of High Performance Liquid Chromatographic (HPLC) Relative Retention Time and Peak Height Ratio to Toxicological Screening

ZHANG YURONG; LIU QI; SUN ZHIQING(INSTITUTE OF FORENSIC SCIENCES; SHANGHAI 200083)   

  • Online:1995-04-25 Published:1995-04-28

被引次数

10

摘要： 本文用高效液相色谱法相对保留时间、２３０ｎｍ和２５０ｎｍ两个紫外检测的峰高比、荧光和２５０ｎｍ紫外检测的峰高比三个指标相结合对毒物进行筛选，３７种毒物区分率由单用保留时间区分的２４．３％提高到９４．６％，文中还考察了各种因素对相对保留时间和峰高比的影响。

关键词: 高效液相色谱法, 相对保留时间, 峰高比, 毒物筛选

Abstract: The method of toxicological screening by HPLC relative retention time, peak height ratio detected by UV 230nm and UV 250nm and peak height ratio detected by fluorescence and ultraviolet 250nm were reported here. Using relative retention time, only 24. 3% of 37 toxicantS could be distinguished. When the retention times, peak height ratio detected by UV 230nm and UV 250nm and peak height ratio detected by fluorescence and UV 250nm were used, 94.6% of 37 toxicants could be distinguished. The influence of concentration and volume in injected sample, the ratio of methanol and pH in mobile phase, different column as well as the column temperature on the relative retentiontime and peak height ratio of toxicants was observed.

Key words: relative retention time, peak height ratio, toxicological screening