法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (6): 450-453.DOI: 10.3969/j.issn.1004-5619.2015.06.009

• 论著 • 上一篇    下一篇

大鼠甲醇中毒后体内的甲酸分布

刘冬梅1，2，周  姝2，陈捷敏2，夏文涛2   

  1. 1. 上海华医司法鉴定所，上海 200335； 2. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063
  • 发布日期:2015-12-25 出版日期:2015-12-28
  • 通讯作者: 陈捷敏，女，副主任法医师，助理研究员，主要从事法医临床学与法医毒理学研究；E-mail：chenjm@ssfjd.cn
  • 作者简介:刘冬梅（1987—），女，硕士，主要从事法医临床学研究；E-mail：l_dm1987@163.com
  • 基金资助:

    上海市自然科学基金资助项目（14ZR1442400）；中央级公益基金资助项目（GY2013Z-1）

Distribution of Formic Acid after Methanol Intoxication in Rats

LIU Dong-mei1,2, ZHOU Shu2, CHEN Jie-min2, XIA Wen-tao2   

  1. 1. INSTITUTE OF HUAYI FORENSIC SCIENCE, SHANGHAI 200335, CHINA; 2. SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE, INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, P.R.CHINA, SHANGHAI 200063, CHINA
  • Online:2015-12-25 Published:2015-12-28
  • Contact: 夏文涛，男，研究员，主任法医师，硕士研究生导师，主要从事法医临床学的鉴定与研究；E-mail：xiawentao629@ 163.com
  • Supported by:
     

摘要： 目的 研究大鼠甲醇中毒后血液及体内主要组织中甲酸的浓度及分布特点。 方法 将SD大鼠分为对照组、中毒3 d组和中毒7 d组，中毒模型按照首剂量8 mL/kg给予大鼠甲醇灌胃，24 h后给予减半剂量4 mL/kg再次灌胃，在首次灌胃后的3 d和7 d将大鼠引颈处死，采心血并提取肝、肾、脑、心和胃组织，利用高效液相色谱仪检测其中的甲酸含量。 结果 甲酸在组织中的浓度高于血液中；与中毒3 d组比较，中毒7 d组脑和胃组织内的甲酸质量浓度有一定的增加趋势，而肝和肾组织中的甲酸质量浓度有所下降（P<0.05）。 结论 高效液相色谱法可以作为一种准确检测甲酸的定性定量方法。大鼠甲醇中毒后，其代谢产物甲酸在血液及组织中均有蓄积，在器官组织中的蓄积更显著。

 

关键词: 法医毒理学, 中毒, 甲醇, 甲酸, 高效液相色谱法, 组织分布

Abstract: Objective To investigate concentration and distribution in blood and tissues of formic acid after methanol intoxication in rats. Methods The Sprague-Dawley rats were divided into groups for control group and 3-day and 7-day intoxication treatment groups. The experimental groups were administered methanol by gavage with the initial dose of 8 mL/kg and followed with 4 mL/kg supplemental dose 24 h later. After 3 days and 7 days later, rats were killed by decapitation. Then samples of cardiac blood, liver, kidney, brain, heart and stomach of each group were collected. Formic acid concentrations were detected by high performance liquid chromatography. Results Formic acid concentrations in tissues were higher than in blood. Compared with 3-day intoxication group, there was an increase formic acid of concentration in brain and stomach in 7-day intoxication group, while a decrease in liver and kidney （P<0.05）. Conclusion High performance liquid chromatography could be used to accurately detect formic acid. As the metabolite of methanol, formic acid accumulates in rat blood and tissues after intoxication and the concentrations in organs and tissues are obviously higher than in blood.

Key words: forensic toxicology, poisoning, methanol, formic acid, high performance liquid chromatography, tissue distribution

中图分类号: