法医学杂志

法医学杂志 ›› 1996, Vol. 0 ›› Issue (3): 0-0.

• 论文 •    

大鼠索沟生活反应的免疫组化研究

陈晓瑞，秦启生，张益鹄，朱少华，赵小红   

  1. 同济医科大学法医学系
  • 发布日期:1996-06-25 出版日期:1996-06-28

Immunohistochemical Study on Vital Reaction of Ligature Mark in Rats

CHEN XIAORUT; QING QISHENG;ZHANG YIHU; ZHU SHAOHUA AND ZHAO XIAOHONG (DEPARTMENT OF FORENSIC MEDICINE, TONG JI MEDICAL UMVERSITY,HUBEI WUHAN 43O030)   

  • Online:1996-06-25 Published:1996-06-28

被引次数

3

摘要： 在４０只Ｗｉｓｔａｔ大鼠颈部制成５分钟至１小时生前索沟损伤模型、应用免疫组化技术（ＳＡＢＣ），观察不同损伤时间索沟常规石蜡切片上纤维连结蛋白（Ｆｎ）和纤维蛋白原（Ｆｂ）的阳性着色和分布情况。结果发现，生前索沟损伤５分钟即能在索沟部真皮组织内发现Ｆｎ和Ｆｂ阳性反应，随着损伤时间的延长，阳性反应加深。Ｆｎ和Ｆｂ最强的着色是带状显于索沟表面表皮剥脱处，除个别死后索沟真皮内有Ｆｂ的弱阳性着色外所有对照组均未见阳性反应。结果表明Ｆｎ和Ｆｂ可以作为生前和死后索沟的鉴别指标。

关键词: 索沟, 生活反应, 纤维联结蛋白, 纤维蛋白原, 免疫组织化学

Abstract: The model of ligature mark injuries (5 min to 1 hr.) were produced on the neck of 40 wistar rats. The positive staining and distribution of Fibronectin (Fn) and Fibrinogen (Fb) in routine paraffin sections of the region of ligature mark were observed using streptuvidin-biotinperoxi-dase complex (SABC) technique. The results showed that a positive reaction of Fn and Fb could be detected in the skin corium under ligature marks only 5 minutes after injury. The longer the injury-time, the stronger positive staining could be observed.The strongest band-sharped positive stining of ligature marks where the epiderm was lost. No positive reaction could be observed in control groups with the exception of a few postmortem ligature marks which a weak akining of Fb in the skin corium could be found. The results indicate that detecting Fn and Fb are useful for distinguishing between atemortem and postmortem ligature mark.

Key words: Ligature mark, Vital reaction, Fibronectin, Fibrinogen, Immunohistochemical