法医学杂志

法医学杂志 ›› 1999, Vol. 0 ›› Issue (2): 0-0.

• 论文 •    

实验性脑挫伤GFAP,PCNA免疫组织化学研究

吴旭,汪德文,张国华,陈怀芳,单亚明,陶陆阳   

  1. 中国医科大学法医学系法医病理教研室,沈阳刑事科学技术研究所
  • 发布日期:1999-04-25 出版日期:1999-04-28

The changes of GFAP,PCNA after brain contusion:an immunohistochemical study in forensic pathology

WU XU,WANG DEWEN,ZHANG GUOHUA,ET AL (DEPARTMENT OF FORENSIC PATHOLOGY, CHINA MEDICAL UNIVERSITY,SHENYANG 110001,P.R.CHINA)   

  • Online:1999-04-25 Published:1999-04-28

被引次数

35

摘要： 采用改进的Ｆｅｅｎｅｙ氏落体打击致Ｗｉｓｔａｒ大鼠脑挫伤模型，利用免疫组织化学染色，观察大鼠实验性脑挫伤后ＧＦＡＰ、ＰＣＮＡ的改变，用图像分析仪对免疫组化染色阳性细胞灰度和面积进行测量，ＳＡＳ统计软件分析，得出脑挫伤后ＧＦＡＰ、ＰＣＮＡ变化的时间规律性，为脑挫伤形成时间推断提供新的手段。ＧＦＡＰ阳性细胞面积和灰度伤后呈上升趋势，伤后３ｈ，即有显著增加（Ｐ＜０．０１），至４天时，阳性细胞灰度，面积达到峰值（Ｐ＜０．０１），伤后７天仍保持较高水平（Ｐ＜０．０１）。ＰＣＮＡ阳性细胞伤后１２ｈ出现，灰度呈上升趋势，面积呈下降趋势，伤后３天时达到最大值（Ｐ＜０．０１）。因此，ＧＦＡＰ、ＰＣＮＡ免疫组织化学染色阳性细胞的数量，灰度，面积改变有时间性规律；推断２～７天的脑挫伤则以ＧＦＡＰ、ＰＣＮＡ改变为主；脑挫伤后星形胶质细胞数目增多，只有很少一部分为星形胶质细胞增殖而来，且这种增殖在伤后３天时达到高峰

关键词: 脑挫伤, 免疫组织化学, 图像分析

Abstract: After the model of experimental brain contusion was set up,the changes of GFAP,PCNA were studied by immunohistochemistry SP method combined with image quantity analysis.After contusion,the gray degrees and areas of GFAP-positive cells increased significantly at the 3rd hour,reached the highest level in the 4th day and kept until the 7th day.PCNA-positive cells appeared in the 12th hour, simultaneously, their gray degrees tended to increase and positive areas tended to decrease. It showed regular changes of GFAP,PCNA with various survival times, and these changes were useful in the diagnosis of brain contusion especially in the period from the second to 7th day after injury.The numbers of astrocytes increased and reached the highest level in the 3rd day after contusion while the reactive proliferation of astrocytes is only a minor phenomenon.

Key words: brain contusion, immunohistochemistry, Glial fibrillary acidic protein