法医学杂志

法医学杂志 ›› 1998, Vol. 0 ›› Issue (1): 14-15+1.

• 论文 • 上一篇    下一篇

HRP标记探针MVR分型法在法医学上的应用

郑秀芬,叶健,倪锦堂,李伯龄   

  1. 公安部物证鉴定中心!北京,100038,公安部物证鉴定中心!北京,100038,公安部物证鉴定中心!北京,100038,公安部物证鉴定中心!北京,100038
  • 发布日期:1998-02-25 出版日期:1998-02-28

Application of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probes

ZHENG XIUFEN, YE JIAN, NI JINTANG, LI BOLING(INSTITUTE OFFORENSIC SCIENCE, P.R.CHINA, BEJING 100038)   

  • Online:1998-02-25 Published:1998-02-28

被引次数

2

摘要： 采用辣根酶标记寡核管酸探针数字编码小卫星MS可变重复序列（HRP标记探针MVR方法），检验微量生物材料。结果表明，相当于5μl血液的血痕、单根有毛囊的毛发以及相当于4μl唾液的斑迹均获得了清晰易读的MVR图谱，灵敏度达1ng。将此方法应用于检案实践取得了较好的效果。MVR－PCR技术在法医学个人识别和亲子鉴定方面极有应用前景。

关键词: MVR－PCR, 辣根酶数字编码, DNA分型, 个人认定

Abstract: The pulPose of this study is to detect small amount ofbiological materials using the method of MVR-PCR atDIS8 locus with HRP-labeled MS32 oligonucleotidesprobe. The results showed that the MVR-PCR patterns,obtained from trace blood stain (equal to 5 ul blood),trace saliva stain (equal to 4 ul saliva) or a hair root, canbe read clearly and easily. The sensitivity of the methodis 1 ng DNA. The applications of the method in personalidentification were discussed.

Key words: D1S8 locus, MVR-PCR, HRP-labeled probe