法医学杂志

法医学杂志 ›› 1998, Vol. 0 ›› Issue (2): 71-73+1.

• 论文 • 上一篇    下一篇

急性心肌缺血早期碱性成纤维细胞生长因子的免疫组化研究

陈建国,徐小虎,赵连旭,吴贤英,祝家镇   

  1. 中山医科大学法医系法医病理学教研室!广东广州,510089,汕头大学医学院病理学教研室!广东汕头,515031,中山医科大学法医系法医病理学教研室!广东广州,510089,汕头大学医学院病理学教研室!广东汕头,515031,中山医科大学法医系法医病理学教研室!广东广州,510089
  • 发布日期:1998-04-25 出版日期:1998-04-28

An immunohistochemical study of basic fibroblast growth factor in early acute myocardial ischcmia

CHEN JIANGUO, XU XIAOHU, ZHAO LIANXU, ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY.SUN YAT-SENUNIVERSITY OF MEDICAL SCIENCES. GUANGZHOU 510089)   

  • Online:1998-04-25 Published:1998-04-28

被引次数

26

摘要： 采用免疫组织化学方法，对大鼠急性心肌缺血早期不同时间心脏不同区域碱性成纤维细胞生长因子basicFibroblastGrowthFactor：bFGF）的变化进行了研究。结果发现心肌缺血30min心肌局部即可出现bFGF阳性表达，并且随着缺血时间的延长其阳性表达的强度也有所增加。图像分析处理结果表明，缺血3h其表达水平达到高峰（55.26±14．75），明显高于对照组（7．27±2．49），而与缺血边缘区域（53．08±14．26）和正常区域(53．79±15．71）相当。3h后，心肌缺血区域bFGF的表达开始出现下降趋势，而缺血边缘区域和正常区域则开始呈现上升趋势。可为心肌缺血的早期诊断提供客观的形态学依据。

关键词: 急性心肌缺血, 成纤维细胞生长因子, 免疫组织化学

Abstract: With the use of immunohistochemistry, the expression of basic fibroblast growth factor (bFGF) in earlyacute myocardial ischemia of rats was studied. It wasobserved that there was an initial (30min) rise in bFGFexpression in limited ischemic myocardium areas andperimyocardial ischemia areas. The grade of expressionof bFGF increased with the continuation of ischemia.The results of image analysis and statistical processsuggested that the expression of bFGF reach a high levelin ischemic myocardial areas after three hour's ofischemia, significantly higher than in control group andin group B1, but no significant difference in pericardialischemia areas and normal areas of the same group. Insummary, early acute myocardial ischemia is accompanied by rapid and prolonged increase in expression ofbFGF with characteristic spatial and temporal kinetic.This finding suggests that the immunohistochemicalstaining of bFGF provide objective morphologicevidence for the diagnosis of early myocardial ischemia.

Key words: acute myocardial ischemia, bFGF, immunohistochemistry