法医学杂志

法医学杂志 ›› 2001, Vol. 0 ›› Issue (1): 7-9+61.

• 论文 • 上一篇    下一篇

补体C_5在心肌梗死死后诊断的特异性研究

胡丙杰,陈玉川,祝家镇,毕启明,李杰,曾家乐,李军   

  1. 中山医科大学法医病理学教研室!广东广州 510089,中山医科大学法医病理学教研室!广东广州 510089,中山医科大学法医病理学教研室!广东广州 510089,中山医科大学法医病理学教研室!广东广州 510089,东莞市公安局法医室!广东 511700,东莞市公安局法医室!广东 511700,中山市公安局
  • 发布日期:2001-02-25 出版日期:2001-02-28

Study on the specificity of complement C_5 for the postmortem diagnosis of myocardial infarction

HU BINGJIE, ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY, SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES, GUANGZHOU,GUANGDONG 510089)   

  • Online:2001-02-25 Published:2001-02-28

摘要： 为了探讨补体 C5在心肌梗死死后诊断的特异性，应用免疫组织化学和图像分析技术 ,对正常心脏、心肌梗死及其它非梗死性的引起直接或间接心脏损害的情况如心肌炎、窒息、电击死、出血性休克、心挫伤、有机磷中毒等心肌细胞内 C5的变化进行研究。结果发现： C5仅在心肌梗死与心肌炎病例出现阳性反应，其阳性反应面积同正常对照组存在显著性差异，在窒息、电击死、出血性休克、心挫伤、有机磷中毒等病例未见明显阳性反应。因此 C5作为心肌梗死死后诊断指标仅受心肌炎的影响，对诊断心肌梗死具有较好的特异性。

关键词: 心肌梗死, 补体, C5, 免疫组织化学, 图像分析, 特异性

Abstract: In order to explore the specificity of complement C5 in the postmortem diagnosis of myocardial infarction, changes of C5 staining in normal, infarcted and other non－ infarcted myocardia with direct or indirect myocardial injuries (myocarditis, mechanical asphyxia, electrocution, hemorrhagic shock, cardiac contusion and organophosphate poisoning) were studied with immunohistochemistry and image analysis. The results showed that positive C5 staining could be observed in groups of myocardial infarction and myocarditis, but not in groups of mechanical asphyxia, electrocution, hemorrhagic shock, cardiac contusion, and organophosphate poisoning. It is indicated that positive reaction of C5 could only be affected by myocarditis, which means that it was more specific for the diagnosis of myocardial infarction.

Key words: myocardial infarction, complement C5, immunohistochemistry, image analysis system, specificity